Biochemical and histological effects of gibberellic acid on Locusta migratoria migratoria fifth instar larvae

被引:17
作者
Abdellaoui, Khemais [1 ]
Ben Halima-Kamel, Monia [1 ]
Acheuk, Fatma [2 ]
Soltani, Noureddine [3 ]
Aribi, Nadia [3 ]
Hamouda, Mohamed HabibBen [1 ]
机构
[1] Univ Sousse, Inst Super Agron Chott Mariem, Dept Sci Biol & Protect Vegetaux, Tunis 4042, Tunisia
[2] Univ Mhamed Bougara, Fac Sci, Dept Biol, Boumerdes 35000, Algeria
[3] Univ Badji Mokhtar, Dept Biol, Lab Biol Anim Appl, Annaba 23000, Algeria
关键词
L; migratoria; Gibberellic acid; Toxicity; Histology; Haemolymph metabolites; RESISTANCE; ORTHOPTERA;
D O I
10.1016/j.pestbp.2013.04.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Experiments were conducted to assess the effect of gibberellic acid (GA(3)), a plant growth regulator, on Locusta migratoria migratoria fifth instar larvae. Newly emerged larvae were exposed to various concentrations of GA(3) administered by topical application or by forced ingestion. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. GA(3) toxicity was also demonstrated by perturbation of the moult processes. In fact, we noted that treated insects present exuviations difficulties due to the impossibility to reject the old integuments causing mortality in the 5th instar larvae. Histological study of proventriculus revealed alterations in the epithelial cells and absence of apolysis phenomenon. Data also showed that GA(3) induced significant quantitative variation of haemolymph metabolites. These changes result in a significant decrease in the total concentration of proteins and carbohydrates and an increase in the total concentration of haemolymph lipids. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:32 / 37
页数:6
相关论文
共 29 条
[1]  
Abdellaoui K., 2009, PLANT PROTECT, V4, P57
[2]  
Abdellaoui K., 2009, Pest Technol, V3, P28
[3]  
Barbouche N., 1986, THESIS TUNISIE
[4]  
Birke A, 2006, J ECON ENTOMOL, V99, P1184, DOI 10.1603/0022-0493-99.4.1184
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]  
DUCHATEAU G, 1959, ARCH INT PHYS BIOCH, V67, P306
[7]   STUDIES ON INSECT ADIPOKINETIC HORMONES [J].
GOLDSWORTHY, GJ ;
GUTHKELCH, J ;
MORDUE, W .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1972, 18 (03) :545-+
[8]  
Greany P. D., 1987, Tropical Science, V27, P261
[9]   IMPROVEMENT IN EFFICACY OF GIBBERELLIC-ACID TREATMENTS IN REDUCING SUSCEPTIBILITY OF GRAPEFRUIT TO ATTACK BY CARIBBEAN FRUIT-FLY [J].
GREANY, PD ;
MCDONALD, RE ;
SCHROEDER, WJ ;
SHAW, PE .
FLORIDA ENTOMOLOGIST, 1991, 74 (04) :570-580
[10]   MANIPULATING AND ENHANCING CITRUS-FRUIT RESISTANCE TO THE CARIBBEAN FRUIT-FLY (DIPTERA, TEPHRITIDAE) [J].
GREANY, PD ;
SHAPIRO, JP .
FLORIDA ENTOMOLOGIST, 1993, 76 (02) :258-263