Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma

被引：13

作者：

Larrinaga, Gorka ^{[1
,2
]}

Sanz, Begona ^{[1
]}

Blanco, Lorena ^{[1
]}

Perez, Itxaro ^{[1
]}

Candenas, Maria L. ^{[3
]}

Pinto, Francisco M. ^{[3
]}

Irazusta, Amaia ^{[2
]}

Gil, Javier ^{[1
]}

Lopez, Jose I. ^{[4
]}

机构：

[1] Univ Basque Country UPV EHU, Fac Med & Dent, Dept Physiol, Leioa 48940, Bizkaia, Spain

[2] Univ Basque Country UPV EHU, Sch Nursing, Dept Nursing 1, Leioa 48940, Bizkaia, Spain

[3] CSIC, Inst Chem Res, E-41080 Seville, Spain

[4] Univ Basque Country UPV EHU, Hosp Univ Cruces, BioCruces Res Inst, Dept Anat Pathol, Baracaldo, Bizkaia, Spain

来源：

CLINICAL BIOCHEMISTRY | 2013年 / 46卷 / 7-8期

关键词：

Chromophobe renal cell carcinoma; Renal oncocytoma; Cannabinoid receptors; CB1; RT-PCR; Immunohistochemistry; TUMOR-GROWTH; ACTIVATION; CANCER; CLASSIFICATION; APOPTOSIS; DIAGNOSIS;

D O I：

10.1016/j.clinbiochem.2012.12.023

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objective: To analyze the mRNA and protein expression of cannabinoid receptors CBI and CB2 in chromophobe renal cell carcinoma (ChRCC) and renal oncocytoma (RO). Design and methods: Fresh and formalin-fixed tissue samples of ChRCC and RO were analyzed by using real-time quantitative RT-PCR and immunohistochemical techniques (n=40). Results: Quantitative RT-PCR analysis showed that CB1 mRNA was underexpressed by 12-fold in ChRCC and had a variable expression in RO. CBI protein showed intense positive immunostaining in both neoplasms. Both CB2 mRNA and protein were not expressed in tumor and non tumor renal tissue. Conclusion: This distinct immunoprofile may eventually be used as an additional tool with practical interest in the differential diagnosis of renal tumors. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：638 / 641

页数：4

共 41 条

[21] 2009 update on the classification of renal epithelial tumors in adults [J].

Lopez-Beltran, Antonio ;

Carrasco, Jose C. ;

Cheng, Liang ;

Scarpelli, Marina ;

Kirkali, Ziya ;

Montironi, Rodolfo .

INTERNATIONAL JOURNAL OF UROLOGY, 2009, 16 (05) :432-443

[22] The CB1/CB2 receptor agonist WIN-55,212-2 reduces viability of human Kaposi's sarcoma cells in vitro [J].

Luca, Tonia ;

Di Benedetto, Giulia ;

Scuderi, Mariagrazia Rita ;

Palumbo, Marco ;

Clementi, Silvia ;

Bernardini, Renato ;

Cantarella, Giuseppina .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2009, 616 (1-3) :16-21

[23] Cannabinoid receptors: Where they are and what they do [J].

Mackie, K. .

JOURNAL OF NEUROENDOCRINOLOGY, 2008, 20 :10-14

[24] Update on the endocannabinoid system as an anticancer target [J].

Malfitano, Anna Maria ;

Ciaglia, Elena ;

Gangemi, Giuseppina ;

Gazzerro, Patrizia ;

Laezza, Chiara ;

Bifulco, Maurizio .

EXPERT OPINION ON THERAPEUTIC TARGETS, 2011, 15 (03) :297-308

[25] STRUCTURE OF A CANNABINOID RECEPTOR AND FUNCTIONAL EXPRESSION OF THE CLONED CDNA [J].

MATSUDA, LA ;

LOLAIT, SJ ;

BROWNSTEIN, MJ ;

YOUNG, AC ;

BONNER, TI .

NATURE, 1990, 346 (6284) :561-564

[26] Cannabinoids in pancreatic cancer:: Correlation with survival and pain [J].

Michalski, Christoph W. ;

Oti, Florian E. ;

Erkan, Mert ;

Saulitmaite, Danguole ;

Bergmann, Frank ;

Pacher, Pal ;

Batkai, Sandor ;

Mueller, Michael W. ;

Giese, Nathalia A. ;

Friess, Helmut ;

Kleeff, Joerg .

INTERNATIONAL JOURNAL OF CANCER, 2008, 122 (04) :742-750

[27] MOLECULAR CHARACTERIZATION OF A PERIPHERAL RECEPTOR FOR CANNABINOIDS [J].

MUNRO, S ;

THOMAS, KL ;

ABUSHAAR, M .

NATURE, 1993, 365 (6441) :61-65

[28] Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus [J].

Pinto, Francisco M. ;

Oscar Pintado, C. ;

Pennefather, Jocelyn N. ;

Patak, Eva ;

Candenas, Luz .

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2009, 7

[29] Endocannabinoid system modulation in cancer biology and therapy [J].

Pisanti, Simona ;

Bifulco, Maurizio .

PHARMACOLOGICAL RESEARCH, 2009, 60 (02) :107-116

[30] Synthetic cannabinoid receptor agonists inhibit tumor growth and metastasis of breast cancer [J].

Qamri, Zahida ;

Preet, Anju ;

Nasser, Mohd W. ;

Bass, Caroline E. ;

Leone, Gustavo ;

Barsky, Sanford H. ;

Ganju, Ramesh K. .

MOLECULAR CANCER THERAPEUTICS, 2009, 8 (11) :3117-3129

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