Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex La(Phth)(phen)3+

被引：14
作者：
Li, L [1 ]
Song, GW [1 ]
Fang, GR [1 ]
机构：
[1] Hubei Univ, Fac Chem & Mat Sci, Wuhan 430062, Peoples R China
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 40卷 / 05期
关键词：
mixed-complex; resonance light-scattering; BSA; aggregation;
D O I：
10.1016/j.jpba.2005.09.004
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A mixed-complex of La(Phth)(phen)(3+) was synthesized. Resonance light-scattering characteristics of interaction between La(Phth)(phen)(3+) with bovine serum albumin (BSA) were studied. When BSA was added, aggregation of La(Phth)(phen)(3+) on the molecular surface of bovine serum albumin occurred in the pH 5.5-6.3, resulted in an enhanced resonance light-scattering (RLS) peak at 360 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：1198 / 1201
页数：4
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