Habenular commissure formation in zebrafish is regulated by the pineal gland-specific gene unc119c

被引：6

作者：

Toyama, Reiko ^{[1
]}

Kim, Mi Ha ^{[1
]}

Rebbert, Martha L. ^{[1
]}

Gonzales, John ^{[2
]}

Burgess, Harold ^{[1
]}

Dawid, Igor B. ^{[1
]}

机构：

[1] NICHD, Program Genom Dev, NIH, Bethesda, MD USA

[2] NICHD, Charles River, NIH, Bethesda, MD USA

来源：

DEVELOPMENTAL DYNAMICS | 2013年 / 242卷 / 09期

关键词：

Zebrafish; pineal gland; epiphysis; habenular commissure; unc119; wnt4; RETINAL DEGENERATION; WNT PROTEINS; TRANSMEMBRANE PROTEIN; NERVOUS-SYSTEM; PHOTORECEPTOR; TRAFFICKING; SECRETION; ABLATION; ELEGANS; NITROREDUCTASE;

D O I：

10.1002/dvdy.23994

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Background: The zebrafish pineal gland (epiphysis) is a site of melatonin production, contains photoreceptor cells, and functions as a circadian clock pacemaker. Since it is located on the surface of the forebrain, it is accessible for manipulation and, therefore, is a useful model system to analyze pineal gland function and development. We previously analyzed the pineal transcriptome during development and showed that many genes exhibit a highly dynamic expression pattern in the pineal gland. Results: Among genes preferentially expressed in the zebrafish pineal gland, we identified a tissue-specific form of the unc119 gene family, unc119c, which is highly preferentially expressed in the pineal gland during day and night at all stages examined from embryo to adult. When expression of unc119c was inhibited, the formation of the habenular commissure (HC) was specifically compromised. The Unc119c interacting factors Arl3l1 and Arl3l2 as well as Wnt4a also proved indispensible for HC formation. Conclusions: We suggest that Unc119c, together with Arl3l1/2, plays an important role in modulating Wnt4a production and secretion during HC formation in the forebrain of the zebrafish embryo. Developmental Dynamics 242: 10331042, 2013. (C) 2013 Wiley Periodicals, Inc.

引用

页码：1033 / 1042

页数：10

共 46 条

[1] RIBEYE recruits munc119, a mammalian ortholog of the Caenorhabditis elegans protein unc119, to synaptic ribbons of photoreceptor Synapses [J].