Ellipsometric and interferometric characterization of DNA probes immobilized on a combinatorial array

被引：87

作者：

Gray, DE ^{[1
]}

CaseGreen, SC ^{[1
]}

Fell, TS ^{[1
]}

Dobson, PJ ^{[1
]}

Southern, EM ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

LANGMUIR | 1997年 / 13卷 / 10期

关键词：

D O I：

10.1021/la962139m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have used ellipsometry to study oligonucleotides bound on an array by observing changes in the optical thickness of the organic material attached to the surface. Interferometry has been used as a complementary technique to confirm our ellipsometry measurements. We have used these optical methods to characterize the chemical steps involved in synthesizing oligonucleotides on a solid support. Large area arrays have been mapped by ellipsometry with a spatial resolution of similar to 1 mm(2) and a sub-nanometer optical thickness resolution. We have demonstrated that this method can differentiate between areas containing oligonucleotides of different lengths.

引用

页码：2833 / 2842

页数：10

共 30 条

[1]

[Anonymous], 1969, 479 NBS

[2]

Azzam R.M.A., 1977, ELLIPSOMETRY POLARIS

[3] A NOVEL METHOD FOR NUCLEIC-ACID SEQUENCE DETERMINATION [J].