Evaluation of a method for the simultaneous detection of multiple tumor markers using a multiplex suspension bead array

被引：10
|
作者：
Wang, Yajie [1 ,2 ,3 ]
Fang, Fang [1 ]
Shi, Congning [1 ]
Zhang, Xulong [4 ]
Liu, Li [2 ]
Li, Jiutong [5 ]
Zhou, Xuelei [5 ]
Yao, Jianer [5 ]
Kang, Xixiong [1 ]
机构：
[1] Capital Med Univ, Beijing Tiantan Hosp, Dept Clin Lab Diag, Beijing 100050, Peoples R China
[2] Capital Med Univ, Beijing Tiantan Hosp, Lab Clin Med Res, Beijing 100050, Peoples R China
[3] Beijing Key Lab Translat Med Cerebrovasc Dis, Beijing, Peoples R China
[4] Capital Med Univ, Sch Basic Med Sci, Dept Immunol, Beijing 100069, Peoples R China
[5] Shanghai Tellgen Life Sci Co Ltd, Shanghai 201203, Peoples R China
来源：
CLINICAL BIOCHEMISTRY | 2012年 / 45卷 / 16-17期
关键词：
Multiplex suspension bead array; Tumor marker; MICROSPHERE IMMUNOASSAY; QUANTITATION; CYTOKINES;
D O I：
10.1016/j.clinbiochem.2012.07.087
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective: To achieve higher tumor detection efficiency, we evaluated a multiplex assay for TM analysis based on the Luminex-100 multiplex suspension bead array. Design: The assay simultaneously determined the concentrations of nine TMs in 1114 human serum specimens (546 patients with tumors, 158 patients with non-tumor inflammatory diseases, and 410 normal controls). The nine TMs were AFP, CEA, CA125, CYFRA 21-1, CA242, f-PSA, t-PSA, NSE and free beta-hCG. The multiplex suspension bead assays were compared with conventional methods used in clinical laboratories. Results: The Luminex assay has the same levels of sensitivity, specificity and accuracy in the prediction of positive tumor specimens as conventional methods. Conclusion: Multiplex suspension bead arrays have promising applications in clinical laboratories. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：1394 / 1398
页数：5
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