Activation kinetics and single channel properties of recombinant alpha(1)beta(2)gamma(2L) GABA(A) receptor channels

被引：17

作者：

Jahn, K

Hertle, I

Bufler, J

Adelsberger, H

Pestel, E

Zieglgansberger, W

Dudel, J

Franke, C

机构：

[1] TECH UNIV MUNICH, DEPT ANESTHESIOL, D-81675 MUNICH, GERMANY

[2] TECH UNIV MUNICH, DEPT PHYSIOL, D-81675 MUNICH, GERMANY

[3] MAX PLANCK INST PSYCHIAT, D-80804 MUNICH, GERMANY

来源：

NEUROREPORT | 1997年 / 8卷 / 16期

关键词：

gamma-aminobutyric acid; GABA(A) receptor channel; HEK; 293; cells; kinetics; patch-clamp; recombinant receptor; single channel; ultra-fast application;

D O I：

10.1097/00001756-199711100-00006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

RECOMBINANT alpha(1) beta(2) gamma(2L) GABA(A) receptor channels, transiently expressed in HEK 293 cells, were investigated using the patch-clamp technique in combination with a device for ultra-fast solution exchange. The dose-response relationship revealed an EC50 of 11.6 +/- 0.9 mu M and saturated with 3 mM GABA. The slope between 0.001 and 0.01 mM GABA was 2.2 +/- 0.4, indicating at least three binding sites for GABA. The rise time decreased from about 120 ms at 0.001 mM GABA to about 0.8 ms at 10 mM GABA. Single channel openings were grouped in bursts with an average duration of 10.3 +/- 3.0 ms. More than 95% of the current was represented by a single channel slope conductance of about 29 pS.

引用

页码：3443 / 3446

页数：4