beta-xylosidase and xylanase characterization and production by Streptomyces sp CH-M-1035

被引:27
作者
Flores, ME
Perez, R
Huitron, C
机构
[1] Department of Biotechnology, Institute of Biomedical Research, National University of Mexico, México, DF
[2] Department of Biotechnology, Institute of Biomedical Research, National University of Mexico, México, 20 DF 04510
关键词
D O I
10.1046/j.1472-765X.1997.00149.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extracellular xylanase activity and cell-bound beta-xylosidase production by a selected strain of Streptomyces sp. CH-M-1035 was characterized during growth on three xylans, sugar cane bagasse pith and lemon peel as sole carbon source. The cell-bound beta-xylosidase and extracellular endoxylanase had pH optima of 6.0 and 5.0, and temperature optima of 50 degrees C and 60 degrees C, respectively. The highest level of beta-xylosidase activity was obtained when Streptomyces sp. CH-M-1035 was grown on larchwood xylan, whereas the maximal endoxylanase production was found on lemon peel. Reducing sugars accumulated in the culture media when Streptomyces sp. CH-M-1035 was grown on xylans, but not on agroindustrial residues.
引用
收藏
页码:410 / 416
页数:7
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