beta-xylosidase and xylanase characterization and production by Streptomyces sp CH-M-1035

Extracellular xylanase activity and cell-bound beta-xylosidase production by a selected strain of Streptomyces sp. CH-M-1035 was characterized during growth on three xylans, sugar cane bagasse pith and lemon peel as sole carbon source. The cell-bound beta-xylosidase and extracellular endoxylanase had pH optima of 6.0 and 5.0, and temperature optima of 50 degrees C and 60 degrees C, respectively. The highest level of beta-xylosidase activity was obtained when Streptomyces sp. CH-M-1035 was grown on larchwood xylan, whereas the maximal endoxylanase production was found on lemon peel. Reducing sugars accumulated in the culture media when Streptomyces sp. CH-M-1035 was grown on xylans, but not on agroindustrial residues.