Characterization of newly emerged NADC30-like strains of porcine reproductive and respiratory syndrome virus in China

被引：48

作者：

Zhang, Hongliang ^{[1
]}

Leng, Chaoliang ^{[2
]}

Ding, Yushan ^{[2
]}

Zhai, Hongyue ^{[2
]}

Li, Zhen ^{[1
]}

Xiang, Lirun ^{[1
]}

Zhang, Wenli ^{[1
]}

Liu, Chunxiao ^{[1
]}

Li, Minhua ^{[1
]}

Chen, Jiazeng ^{[4
]}

Bai, Yun ^{[4
]}

Kan, Yunchao ^{[2
]}

Yao, Lunguang ^{[2
]}

Peng, Jinmei ^{[1
]}

Wang, Qian ^{[1
]}

Tang, Yan-Dong ^{[1
]}

An, Tongqing ^{[1
]}

Cai, Xuehui ^{[1
]}

Tian, Zhijun ^{[1
]}

Tong, Guangzhi ^{[3
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China

[2] Nanyang Normal Univ, Henan Key Lab Insect Biol Funiu Mt, Henan Prov Engn Lab Insects Bioreactor, China UK NYNU RRes Joint Lab Insect Biol, 1638 Wolong Rd, Nanyang 473061, Peoples R China

[3] Chinese Acad Agr Sci, Shanghai Vet Res Inst, 518 Ziyue Rd, Shanghai 200241, Peoples R China

[4] Shandong Sinder Technol Co Ltd, Qingdao 266100, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2019年 / 164卷 / 02期

基金：

中国国家自然科学基金;

关键词：

NONSTRUCTURAL PROTEIN-2; MAINLAND CHINA; PATHOGENICITY; VACCINES; DISEASE;

D O I：

10.1007/s00705-018-4080-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Different strains of porcine reproductive and respiratory syndrome virus (PRRSV) have emerged and circulated in different regions of mainland China since 1996, particularly after 2006. In 2012, NADC30-like PRRSV was first isolated in Henan Province. By 2016, it had spread to most provinces in China. In the present study, the whole genomes (excluding the poly(A) tails) of 13 newly emerged NADC30-like PRRSV strains were sequenced and analyzed. Furthermore, the pathogenicity of SD53-1603, one of the 13 PRRSV strains, was assessed. Phylogenetic analysis showed that these 13 newly emerged NADC30-like PRRSV strains, together with some reference strains, formed a new subgroup (subgroup 5), characterized by a predicted 131-amino-acid deletion in the nonstructural protein (NSP) 2. However, low levels of whole-genome similarity and a wide variety of recombination patterns complicated the classification of the NADC30-like PRRSV isolates. Interestingly, almost all of the recombination breakpoints found in these 13 PRRSV isolates and other NADC30-like PRRSV isolates occurred in genes encoding NSPs and/or minor structural proteins. In addition, piglets infected with the newly emerged NADC30-like strain SD53-1603 displayed clear clinical respiratory symptoms and underwent typical pathological changes. The findings may be useful for elucidating the characteristics and epidemic status of NADC30-like PRRSV in China.

引用

页码：401 / 411

页数：11

共 38 条

[1] Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: Coexistence of two NA-subgenotypes with great diversity [J].