Identification of three distinct regions of allelic deletions on the short arm of chromosome 8 in hepatocellular carcinoma

被引:99
作者
Pineau, P
Nagai, H
Prigent, S
Wei, Y
Gyapay, G
Weissenbach, J
Tiollais, P
Buendia, MA
Dejean, A
机构
[1] Inst Pasteur, INSERM, Unite Recombinaison & Express Genet, U163, F-75724 Paris 15, France
[2] Genethon, CNRS, URA 1922, F-91000 Evry, France
关键词
hepatocellular carcinoma; chromosome; 8p; loss of heterozygosity; tumor suppressor gene; microsatellite marker;
D O I
10.1038/sj.onc.1202648
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chromosome 8p is associated with a large number of allelic imbalances in epithelial tumors including hepatocellular carcinoma (HCC), However, no tumor suppressor gene has been identified so far in this particular region of the genome. To further clarify the pattern of allelic deletions on chromosome 8p in HCC, we have undertaken high-density polymorphic marker analysis of 109 paired normal and primary tumor samples using 40 microsatellites positioned every 2 cm in average throughout 8p, We found that 60% of the tumors exhibited loss of heterozygosity (LOH) at one or more loci at 8p with three distinct minimal deleted areas : a 13 cm region in the distal part of 8p21, a 9 cm area in the more proximal portion of 8p22 and a 5 cm area in 8p23, These data strongly suggest the presence of at least three novel tumor suppressor loci on 8p in hepatocellular carcinoma.
引用
收藏
页码:3127 / 3134
页数:8
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