Serological evidence of an Australian bovine lentivirus

被引：17

作者：

Burkala, EJ ^{[1
]}

Ellis, TM

Voigt, V

Wilcox, GE

机构：

[1] Murdoch Univ, Dept Vet & Biomed Studies, Murdoch, WA 6150, Australia

[2] Agr WA, Anim Hlth Labs, S Perth, WA 6151, Australia

来源：

VETERINARY MICROBIOLOGY | 1999年 / 68卷 / 1-2期

关键词：

bovine immunodeficiency virus; BIV; Jembrana disease virus; bovine lentivirus; Australia; recombinant protein; seroprevalence;

D O I：

10.1016/S0378-1135(99)00073-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant 26 kDa capsid (CA) proteins of bovine lentiviruses, bovine immunodeficiency virus (BIV) and Jembrana disease virus (JDV), were expressed in Escherichia coli and utilised as antigens for an enzyme-linked immunosorbent assay (ELISA) and a western immunoblot (WIB) procedure for the detection of antibody in dairy cattle in Western Australia. A total of 690 serum samples, 30 from each of 23 farms, were tested by ELISA with a JDV CA protein antigen, and antibody was detected in 3.8% (p < 0.05) of the sera. Nine sera from each farm were also tested by WIB with JDV CA protein antigens and antibody was detected in 15.9% of these samples. All ELISA-positive results were also WIB-positive, and all sera antibody-positive by WIB with JDV CA protein antigens were also antibody-positive by the WIB using recombinant BIV CA antigens. This study showed that recombinant protein antigens can be used for serological tests to detect bovine lentivirus infection in Australia. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 177

页数：7

共 26 条

[1]

Belloc C, 1996, VET RES, V27, P395

[2]

Black JW, 1989, P US ANIM HLTH ASS, V93, P145

[3] Recombinant Jembrana disease virus proteins as antigens for the detection of antibody to bovine lentiviruses [J].

Burkala, EJ ;

Narayani, I ;

Hartaningsih, N ;

Kertayadnya, G ;

Berryman, DI ;

Wilcox, GE .

JOURNAL OF VIROLOGICAL METHODS, 1998, 74 (01) :39-46

[4] CHARACTERIZATION OF EARLY PATHOGENIC EFFECTS AFTER EXPERIMENTAL-INFECTION OF CALVES WITH BOVINE IMMUNODEFICIENCY-LIKE VIRUS [J].

CARPENTER, S ;

MILLER, LD ;

ALEXANDERSEN, S ;

WHETSTONE, CA ;

VANDERMAATEN, MJ ;

VIUFF, B ;

WANNEMUEHLER, Y ;

MILLER, JM ;

ROTH, JA .

JOURNAL OF VIROLOGY, 1992, 66 (02) :1074-1083

[5] NUCLEOTIDE-SEQUENCE ANALYSIS OF JEMBRANA DISEASE VIRUS - A BOVINE LENTIVIRUS ASSOCIATED WITH AN ACUTE DISEASE SYNDROME [J].

CHADWICK, BJ ;

COELEN, RJ ;

WILCOX, GE ;

SAMMELS, LM ;

KERTAYADNYA, G .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :1637-1650

[6]

COATS KSC, 1995, VET REC, V136, P269

[7] SEROPREVALENCE OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS AND BOVINE LEUKEMIA-VIRUS IN A DAIRY-CATTLE HERD [J].

COCKERELL, GL ;

JENSEN, WA ;

ROVNAK, J ;

ENNIS, WH ;

GONDA, MA .

VETERINARY MICROBIOLOGY, 1992, 31 (2-3) :109-116

[8] STUDIES ON EXPERIMENTAL JEMBRANA DISEASE IN BALI CATTLE .3. PATHOLOGY [J].

DHARMA, DMN ;

BUDIANTONO, A ;

CAMPBELL, RSF ;

LADDS, PW .

JOURNAL OF COMPARATIVE PATHOLOGY, 1991, 105 (04) :397-414

[9] SEROLOGICAL EVIDENCE FOR THE PRESENCE OF BOVINE LENTIVIRUS INFECTION IN CATTLE IN AUSTRALIA [J].

FORMAN, AJ ;

GIBSON, CA ;

RODWELL, BJ .

AUSTRALIAN VETERINARY JOURNAL, 1992, 69 (12) :337-337

[10] ANTIBODY-RESPONSE TO JEMBRANA DISEASE VIRUS IN BALI CATTLE [J].

HARTANINGSIH, N ;

WILCOX, GE ;

KERTAYADNYA, G ;

ASTAWA, M .

VETERINARY MICROBIOLOGY, 1994, 39 (1-2) :15-23

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