Effect of fsDNA on chemiluminescence characteristics of luminol-H2O2 system catalyzed by Mn(III)-Tetrakis (4-sulfonatophenyl)-porphyrin

被引：9

作者：

Kazemi, Sayed Yahya ^{[1
]}

Abedirad, Seyed Mohammad ^{[1
]}

机构：

[1] Sari Agr Sci & Nat Resources Univ, Dept Basic Sci, Sari, Iran

来源：

JOURNAL OF THE IRANIAN CHEMICAL SOCIETY | 2013年 / 10卷 / 02期

关键词：

Quenching chemiluminescence; Kinetic profile; DNA; Stern-Volmer plot; Manganese tetrasulfonatophenyl porphyrin; HYDROGEN-PEROXIDE; DNA; PORPHYRIN; ASSAY; KINETICS;

D O I：

10.1007/s13738-012-0153-9

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present work, the effect of fsDNA (fish sperm DNA) on kinetic parameters of chemiluminescence (CL) of the luminol-hydrogen peroxide system catalyzed by Mn(III)-Tetrakis (4-sulfonatophenyl)-porphyrin was investigated. These parameters including pseudo first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL were evaluated by computer fitting of the resulted intensity-time plots. Results reveal that CL parameters are dramatically affected due to interaction of metalloporphyrin with DNA. In order to observe quenching effect of DNA on CL intensity, Stern-Volmer plot with k (Q) value of 1.18 x 10(5) M-1 was calculated in the quencher concentration range of 4 x 10(-6)-8.5 x 10(-5) M.

引用

页码：251 / 256

页数：6

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