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Differential chemokine and cytokine production by neonatal bovine γδ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection
被引:45
作者:
McGill, Jodi L.
[1
]
Nonnecke, Brian J.
[1
]
Lippolis, John D.
[1
]
Reinhardt, Timothy A.
[1
]
Sacco, Randy E.
[1
]
机构:
[1] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA
来源:
关键词:
bovine gamma delta T cells;
respiratory syncytial virus;
toll-like receptors;
vitamin D;
VITAMIN-D;
GENE-EXPRESSION;
LYMPHOCYTE SUBSETS;
IMMUNE-SYSTEM;
ANAPLASMA-MARGINALE;
SURFACE-MOLECULE;
DISEASE;
WC1;
ANTIGEN;
CALVES;
D O I:
10.1111/imm.12075
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
T cells respond to stimulation via toll-like receptors (TLR). Bovine T cells express TLR3 and TLR7, receptors that are key for the recognition of viruses such as bovine respiratory syncytial virus (BRSV); however, responses of T cells to stimulation via these receptors, and their role during viral infections, remains unclear. Here, we demonstrate that neonatal bovine T cells exhibit robust chemokine and cytokine production in response to the TLR3 agonist, Poly(I:C), and the TLR7 agonist, Imiquimod. Importantly, we observe a similar phenotype in T-cell subsets purified from calves infected with BRSV. Bovine T cells are divided into subsets based upon their expression of WC1, and the response to TLR stimulation and viral infection differs between these subsets, with WC1.1+ and WC1neg T cells producing macrophage inflammatory protein-1 and granulocytemacrophage colony-stimulating factor, and WC1.2+ T cells preferentially producing the regulatory cytokines interleukin-10 and transforming growth factor-. We further report that the active vitamin D metabolite 1,25-dihydroxyvitamin D3 does not alter T-cell responses to TLR agonists or BRSV. To our knowledge, this is the first characterization of the T-cell response during in vivo BRSV infection and the first suggestion that WC1.1+ and WC1neg T cells contribute to the recruitment of inflammatory populations during viral infection. Based on our results, we propose that circulating T cells are poised to rapidly respond to viral infection and suggest an important role for T cells in the innate immune response of the bovine neonate.
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页码:227 / 244
页数:18
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