Antiblood Cell Adhesion of Mussel-Inspired Chondroitin Sulfate- and Caffeic Acid-Modified Polycarbonate Membranes

被引：2

作者：

Krishnamoorthi, Rajakumari ^{[1
,2
]}

Anbazhagan, Rajeshkumar ^{[1
,2
]}

Thankachan, Darieo ^{[3
]}

Dinh, Van Thi Thuy ^{[1
,2
]}

Tsai, Hsieh-Chih ^{[1
,2
,4
]}

Lai, Juin-Yih ^{[1
,2
,4
,5
]}

Wang, Chih-Feng ^{[1
,2
,4
]}

机构：

[1] Natl Taiwan Univ Sci & Technol, Grad Inst Appl Sci & Technol, Taipei 106, Taiwan

[2] Natl Taiwan Univ Sci & Technol, Adv Membrane Mat Ctr, Taipei 106, Taiwan

[3] Natl Taiwan Univ Sci & Technol, Dept Mat Sci & Engn, Taipei 106, Taiwan

[4] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Taoyuan 320, Taiwan

[5] Yuan Ze Univ, Dept Chem Engn & Mat Sci, Taoyuan 320, Taiwan

来源：

LANGMUIR | 2022年 / 39卷 / 02期

关键词：

CROSS-LINKING; HEPARIN; HYDROGEL; HEMOCOMPATIBILITY; POLYELECTROLYTE; OSTEOARTHRITIS; NANOPARTICLES; SUBSTITUTION; ADSORPTION; POLYMERS;

D O I：

10.1021/acs.langmuir.2c01688

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We fabricated a mussel-inspired hemocompatible polycarbonate membrane (PC) modified by the cross-linking of chondroitin sulfate and caffeic acid polymer using CA -CS via a Schiff base and Michael addition reaction and named it CA-CS-PC. The as -fabricated CA-CS-PC membrane shows excellent hydrophilicity with a water contact angle of 0 degrees and a negative surface charge with a zeta potential of -32 mV. The antiadhesion property of the CA-CS-modified PC membrane was investigated by enzyme-linked immunosorbent assay (ELISA), using human plasma protein fibrinogen adsorption studies, and proved to have excellent antiadhesion properties, because of the lower fibrinogen adsorption. In addition, the CA-CS-PC membrane also shows enhanced hemocompatibility. Finally, blood cell attachment tests of the CA-CS-PC membrane were observed by CLSM and SEM, and the obtained results proved that CA-CS-PC effectively resisted cell adhesion, such as platelets and leucocytes. Therefore, this work disclosed a new way to design a simple and versatile modification of the membrane surface by caffeic acid and chondroitin sulfate and apply it for cell adhesion.

引用

页码：717 / 727

页数：11