The subgenus-specific C-terminal region of protein IX is located on the surface of the adenovirus capsid

被引：29

作者：

Akalu, A

Liebermann, H

Bauer, U

Granzow, H

Seidel, W

机构：

[1] Univ Greifswald, Inst Med Microbiol, Abt Virol, D-17487 Greifswald, Germany

[2] Fed Res Ctr Virus Dis Anim, Inst Appl Virol, Friedrich Loeffler Inst, D-17498 Insel Riems, Germany

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 07期

关键词：

D O I：

10.1128/JVI.73.7.6182-6187.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have investigated the antigenicity of the C- and N-terminal halves of pIX of human adenovirus types 2 and 3 (Ad2 and Ad3) as well as their orientations in virions. We found that only the C-terminal halves of Ad2 pIX and Ad3 pIX reacted in a subgenus-specific manner by enzyme-linked immunosorbent assay and immunoblot analysis. Based an immunoelectron microscopy experiments, pIX in viral capsids appears to be positioned such that the C-terminaI part of pIX constitutes the surface domain whereas the N terminus of the protein makes up the internal domain in icosahedral Ad capsids.

引用

页码：6182 / 6187

页数：6

共 38 条

[1] Rapid identification of subgenera of human adenovirus by serological and PCR assays [J].