Evaluation of oligosaccharide methods for carbohydrate analysis in a fully human monoclonal antibody and comparison of the results to the monosaccharide composition determination by a novel calculation

被引:16
作者
Adamo, Michael [1 ]
Qiu, Difei [1 ]
Dick, Lawrence W., Jr. [1 ]
Zeng, Ming [1 ]
Lee, An-Horng [1 ]
Cheng, Kuang-Chuan [1 ]
机构
[1] Medarex Inc, Dept Analyt Dev, Bloomsbury, NJ 08804 USA
关键词
Monosaccharides; Oligosaccharides; LC-MS; CE-LIF; HPAEC-PAD; N-LINKED OLIGOSACCHARIDES; TANDEM MASS-SPECTROMETRY; CAPILLARY-ELECTROPHORESIS; POSTTRANSLATIONAL MODIFICATIONS; LIQUID-CHROMATOGRAPHY; VARIABLE REGION; IGG1; ANTIBODIES; GLYCOSYLATION; FC; CHAINS;
D O I
10.1016/j.jpba.2008.10.008
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Carbohydrates can change a drug's properties including solubility, affinity towards antigen, pharmacokinetics and pharmacodynamics. Due to this importance, carbohydrate composition is utilized as a parameter to evaluate a drug candidate's quality. In this study, the compositional monosaccharides of a drug candidate are measured by HPAEC-PAD, while the oligosaccharides are studied by HPAEC-PAD, CE-LIF and LC-MS. The advantages and limitations of these various approaches for oligosaccharide analysis are reviewed in this work. While the methods used for oligosaccharide analysis are well established we have devised a new and novel calculation for determining monosaccharide content using the relative percentages of the N-glycans. This calculation was used to evaluate the accuracy of the oligosaccharide determination methods by comparison of the N-glycan data to the experimental monosaccharide data. The results obtained from this novel calculation demonstrate that the relative abundance of carbohydrates as determined from these various approaches are consistent. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:181 / 192
页数:12
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