The diagnostic methods in the COVID-19 pandemic, today and in the future

被引:480
作者
Wu, So Yat [1 ]
Yau, Hoi Shan [1 ]
Yu, Man Yee [1 ]
Tsang, Hin Fung [1 ]
Chan, Lawrence Wing Chi [1 ]
Cho, William Chi Shing [2 ]
Shing Yu, Allen Chi [3 ]
Yuen Yim, Aldrin Kay [3 ]
Li, Marco J. W. [3 ]
Wong, Yin Kwan Evelyn [1 ]
Pei, Xiao Meng [1 ]
Cesar Wong, Sze Chuen [1 ]
机构
[1] Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Hong Kong, Peoples R China
[2] Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China
[3] Codex Genet Ltd, Hong Kong, Peoples R China
关键词
COVID-19; SARS-CoV-2; coronavirus Disease-2019; severe acute respiratory syndrome coronavirus 2; diagnosis; CORONAVIRUS; SALIVA; ASSAYS;
D O I
10.1080/14737159.2020.1816171
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Introduction: The emergence of anovel coronavirus identified in patients with unknown cause of acute respiratory disease in Wuhan, China at the end of 2019 has caused aglobal outbreak. The causative coronavirus was later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the disease caused by SARS-CoV-2 was named as Coronavirus Disease-2019 (COVID-19). As of 10 August 2020, more than 19,718,030 confirmed cases and 728,013 deaths have been reported. COVID-19 is spread via respiratory droplets which are inhaled into the lungs. Areas covered: In this article, we summarized the knowledge about the causative pathogen of COVID-19 and various diagnostic methods in this pandemic for better understanding of the limitations and the nuances of virus testing for COVID-19. Expert opinion: In this pandemic, rapid and accurate identification of COVID-19 patients are critical to break the chain of infection in the community. RT-PCR provides a rapid and reliable identification of SARS-CoV-2 infection. In the future, molecular diagnostics will still be the gold standard and next-generation sequencing can help us to understand more on the pathogenesis and detect novel mutations. It is believed that more sophisticated detection methods will be introduced to detect SARS-CoV-2 as earliest as possible.
引用
收藏
页码:985 / 993
页数:9
相关论文
共 58 条
[1]   Saliva is a reliable tool to detect SARS-CoV-2 [J].
Azzi, Lorenzo ;
Carcano, Giulio ;
Gianfagna, Francesco ;
Grossi, Paolo ;
Dalla Gasperina, Daniela ;
Genoni, Angelo ;
Fasano, Mauro ;
Sessa, Fausto ;
Tettamanti, Lucia ;
Carinci, Francesco ;
Maurino, Vittorio ;
Rossi, Agostino ;
Tagliabue, Angelo ;
Baj, Andreina .
JOURNAL OF INFECTION, 2020, 81 (01) :E45-E50
[2]  
Centers for Disease Control and Prevention, 2020, COR DIS 2019 LAB
[3]  
Cepheid, 2020, XPERTR XPRESS SARS C
[4]   Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens [J].
Chan, Jasper Fuk-Woo ;
Yip, Cyril Chik-Yan ;
To, Kelvin Kai-Wang ;
Tang, Tommy Hing-Cheung ;
Wong, Sally Cheuk-Ying ;
Leung, Kit-Hang ;
Fung, Agnes Yim-Fong ;
Ng, Anthony Chin-Ki ;
Zou, Zijiao ;
Tsoi, Hoi-Wah ;
Choi, Garnet Kwan-Yue ;
Tam, Anthony Raymond ;
Cheng, Vincent Chi-Chung ;
Chan, Kwok-Hung ;
Tsang, Owen Tak-Yin ;
Yuen, Kwok-Yung .
JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (05)
[5]  
Chan JFW., 2020, EMERG MICROBES INFEC, V9, p221
[6]   Gastrointestinal Manifestations of SARS-CoV-2 Infection and Virus Load in Fecal Samples From a Hong Kong Cohort: Systematic Review and Meta-analysis [J].
Cheung, Ka Shing ;
Hung, Ivan F. N. ;
Chan, Pierre P. Y. ;
Lung, K. C. ;
Tso, Eugene ;
Liu, Raymond ;
Ng, Y. Y. ;
Chu, Man Y. ;
Chung, Tom W. H. ;
Tam, Anthony Raymond ;
Yip, Cyril C. Y. ;
Leung, Kit-Hang ;
Fung, Agnes Yim-Fong ;
Zhang, Ricky R. ;
Lin, Yansheng ;
Cheng, Ho Ming ;
Zhang, Anna J. X. ;
To, Kelvin K. W. ;
Chan, Kwok-H ;
Yuen, Kwok-Y ;
Leung, Wai K. .
GASTROENTEROLOGY, 2020, 159 (01) :81-95
[7]   Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia [J].
Chu, Daniel K. W. ;
Pan, Yang ;
Cheng, Samuel M. S. ;
Hui, Kenrie P. Y. ;
Krishnan, Pavithra ;
Liu, Yingzhi ;
Ng, Daisy Y. M. ;
Wan, Carrie K. C. ;
Yang, Peng ;
Wang, Quanyi ;
Peiris, Malik ;
Poon, Leo L. M. .
CLINICAL CHEMISTRY, 2020, 66 (04) :549-555
[8]   Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].
Corman, Victor M. ;
Landt, Olfert ;
Kaiser, Marco ;
Molenkamp, Richard ;
Meijer, Adam ;
Chu, Daniel K. W. ;
Bleicker, Tobias ;
Bruenink, Sebastian ;
Schneider, Julia ;
Schmidt, Marie Luisa ;
Mulders, Daphne G. J. C. ;
Haagmans, Bart L. ;
van der Veer, Bas ;
van den Brink, Sharon ;
Wijsman, Lisa ;
Goderski, Gabriel ;
Romette, Jean-Louis ;
Ellis, Joanna ;
Zambon, Maria ;
Peiris, Malik ;
Goossens, Herman ;
Reusken, Chantal ;
Koopmans, Marion P. G. ;
Drosten, Christian .
EUROSURVEILLANCE, 2020, 25 (03) :23-30
[9]  
FIND, 2020, SARS COV 2 DIAGN PIP
[10]  
Food and Drug Administration US, COR DIS 2019 COVID 1