High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

被引：60
作者：
Laranjo, M
Machado, J
Young, JPW
Oliveira, S [1 ]
机构：
[1] Univ Evora, ICAM, Lab Microbiol Solo, Evora, Portugal
[2] Univ Evora, Dept Biol, P-7002554 Evora, Portugal
[3] Inst Nacl Saude Dr Ricardo Jorge, Bacteriol Lab, Lisbon, Portugal
[4] Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England
来源：
FEMS MICROBIOLOGY ECOLOGY | 2004年 / 48卷 / 01期
关键词：
Mesorhizobium; 16S-rDNA; DAPD; chickpea; diversity; microbial;
D O I：
10.1016/j.femsec.2003.12.015
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations. 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：101 / 107
页数：7
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