High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

被引：60

作者：

Laranjo, M

Machado, J

Young, JPW

Oliveira, S ^{[1
]}

机构：

[1] Univ Evora, ICAM, Lab Microbiol Solo, Evora, Portugal

[2] Univ Evora, Dept Biol, P-7002554 Evora, Portugal

[3] Inst Nacl Saude Dr Ricardo Jorge, Bacteriol Lab, Lisbon, Portugal

[4] Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England

来源：

FEMS MICROBIOLOGY ECOLOGY | 2004年 / 48卷 / 01期

关键词：

Mesorhizobium; 16S-rDNA; DAPD; chickpea; diversity; microbial;

D O I：

10.1016/j.femsec.2003.12.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations. 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：101 / 107

页数：7

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[11] MEASURES OF THE AMOUNT OF ECOLOGIC ASSOCIATION BETWEEN SPECIES [J].