STAPHYLOCOCCUS AUREUS ENDORIBONUCLEASE III: PURIFICATION AND PROPERTIES

被引：19

作者：

Chevalier, Clement ^{[1
]}

Huntzinger, Eric ^{[1
,2
]}

Fechter, Pierre ^{[1
]}

Boisset, Sandrine ^{[3
]}

Vandenesch, Francois ^{[3
]}

Romby, Pascale ^{[1
]}

Geissmann, Thomas ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, IBMC, Architecture & Reactivite ARN, Strasbourg, France

[2] Max Planck Inst Dev Biol, Tubingen, Germany

[3] Univ Lyon 1, Ctr Natl Reference Staphylocoques, U851, INSERM, F-69365 Lyon, France

来源：

RNA TURNOVER IN BACTERIA, ARCHAEA AND ORGANELLES | 2008年 / 447卷

关键词：

D O I：

10.1016/S0076-6879(08)02216-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcus aureus ribonuclease III (Sa-RNase III) belongs to the enzyme family known to process double-stranded RNAs consisting of two turns of the RNA helix. Although the enzyme is thought to play a role in ribosomal RNA processing and gene regulation, the deletion of the me gene in S. aureus does not affect cell growth in rich medium. S. aureus RNase III acts in concert with regulatory RNAIII to repress the expression of several mRNAs encoding virulence factors. The action of the RNase is most likely to initiate the degradation of repressed mRNAs leading to an irreversible repression. In this chapter, we describe the overexpression and purification of recombinant RNase III from S. aureus, and we show that its biochemical properties are similar to the orthologous enzyme from Escherichia coli. Both enzymes similarly recognize and cleave different RNA substrates and RNA-mRNA duplexes.

引用

页码：309 / 327

页数：19

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