Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

被引：29

作者：

Carlini, Lina ^{[1
]}

Manley, Suliana ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Expt Biophys, Lausanne, Switzerland

来源：

ACS CHEMICAL BIOLOGY | 2013年 / 8卷 / 12期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

LIVING CELLS; FLUORESCENCE; MICROSCOPY; MITOCHONDRIAL; MEMBRANE; RESOLUTION; FLUOROPHORES; ORGANELLES; BLINKING; SYSTEM;

D O I：

10.1021/cb400467x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several live-cell compatible imaging media and evaluated their performance in situ. We identify a number of new dyes and imaging medium-dye combinations for live staining, that densely highlight intracellular structures with excellent photophysical performance for SR.

引用

页码：2643 / 2648

页数：6

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