A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability

被引：584

作者：

Ishiyama, M ^{[1
]}

Miyazono, Y ^{[1
]}

Sasamoto, K ^{[1
]}

Ohkura, Y ^{[1
]}

Ueno, K ^{[1
]}

机构：

[1] DOJINDO LABS,KUMAMOTO 86122,JAPAN

来源：

TALANTA | 1997年 / 44卷 / 07期

关键词：

cell viability; formazan; lactic acid; lactate dehydrogenase; tetrazolium salt; water solubility;

D O I：

10.1016/S0039-9140(97)00017-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly water soluble disulfonated tetrazolium salt, 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt, was synthesized. The compound is reduced by NADH in good yields al neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. The tetrazolium salt thus proved to be useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator. (C) 1997 Elsevier Science B.V.

引用

页码：1299 / 1305

页数：7

共 10 条

[1]

ISHIYAMA M, 1993, CHEM PHARM BULL, V41, P1118, DOI 10.1248/cpb.41.1118

[2] NOVEL DISULFONATED TETRAZOLIUM SALT THAT CAN BE REDUCED TO A WATER-SOLUBLE FORMAZAN AND ITS APPLICATION TO THE ASSAY OF LACTATE-DEHYDROGENASE [J].