Imaging of skin dermal thermal damage by multiphoton autofluroescence and second harmonic generation (SHG) microscopy

被引：0

作者：

Yang, Tsung-Lin ^{[1
]}

Chiang, Cheng-Tien ^{[1
]}

Kao, Hslen-Ching ^{[1
]}

Lo, Wen ^{[1
]}

Lin, Sung-Jan ^{[2
]}

Jee, Shiou-Hwa ^{[2
]}

Chen, Yang-Fang ^{[1
]}

Dong, Chen-Yuan ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Phys, Taipei 10764, Taiwan

[2] Natl Taiwan Univ Hosp & Coll Med, Dept Dermatol, Taipei, Taiwan

来源：

PHOTONIC THERAPEUTICS AND DIAGNOSTICS II | 2006年 / 6078卷

关键词：

multiphoton imaging; autofluorescence; second harmonic generation; elastin; collagen; thermal damage;

D O I：

10.1117/12.647406

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Multiphoton autofluorescence and second harmonic generation (SHG) microscopy are useful in respectively identifying elastin and collagen fibers within the skin dermis. In this study, we attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize the thermal changes to collagen and elastin fibers. We found that autofluorescence and SHG imaging behave differently in skin dermis treated with different temperatures and that an index reflecting the relative changes in autofluorescence and SHG intensity is useful to identify the degree of dermal thermal damage to the skin. With additional development, our approach can be used to identify the extent of thermal damage in patients.

引用

页数：9

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