Non-covalent Nanocomplexes of Folic Acid and Reducible Polyethylenimine for Survivin siRNA Delivery

被引：0

作者：

Zheng, Bin ^{[1
]}

Yang, Shuang ^{[1
]}

Wang, Mengqiao ^{[1
]}

Yang, Xuewei ^{[1
]}

Teng, Lirong ^{[1
]}

Xie, Jing ^{[1
]}

Teng, Lesheng ^{[1
]}

Lee, Robert J. ^{[1
,2
]}

机构：

[1] Jilin Univ, Coll Life Sci, Changchun 130012, Peoples R China

[2] Ohio State Univ, Coll Pharm, Columbus, OH 43210 USA

来源：

ANTICANCER RESEARCH | 2015年 / 35卷 / 10期

关键词：

Polyethylenimine; siRNA; folate receptor; drug delivery; cancer; nanoparticle; GROWTH IN-VITRO; DOWN-REGULATION; GENE DELIVERY; NANOPARTICLES; CONJUGATE; PEI;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Efficient delivery of siRNA is critical for its therapeutic applications. This study was aimed at the design, synthesis and evaluation of a novel delivery system based on non-covalent complexes of folic acid (FA) and a reducible polyethylenimine (PEI) derivative, PEI-SS. Materials and Methods: PEI-SS was synthesized by crosslinking low-molecular weight PEI using a reducible crosslinking agent. PEI-SS-siRNA complexes were synthesized and further combined with FA to form FA/PEI-SS-siRNA nanocomplexes. These were then evaluated in two tumor cell lines for survivin siRNA delivery. Results: FA/PEI-SS-siRNA complexes were taken-up by both HeLa and A549 cells efficiently. This was not affected by the expression level of the folate receptor on the tumor cell surface. Furthermore, FA/PEI-SS-siRNA complexes reduced the level of survivin expression in both cell lines. Conclusion: FA/PEI-SS is a potent siRNA carrier that warrants further evaluation.

引用

页码：5433 / 5441

页数：9

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