Use of a Molecular Genetic Platform Technology to Produce Human Wnt Proteins Reveals Distinct Local and Distal Signaling Abilities

被引：14

作者：

Green, Jennifer L. ^{[1
]}

Bauer, Matthieu ^{[2
]}

Yum, Kyu Won ^{[1
]}

Li, Yao-Cheng ^{[1
]}

Cox, Miranda L. ^{[1
]}

Willert, Karl ^{[2
]}

Wahl, Geoffrey M. ^{[1
]}

机构：

[1] Salk Inst Biol Studies, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA

来源：

PLOS ONE | 2013年 / 8卷 / 03期

关键词：

ACUTE LYMPHOBLASTOID LEUKEMIA; HEMATOPOIETIC STEM-CELLS; WNT/BETA-CATENIN; PATHWAY; RECEPTORS; LGR5; TRANSFORMATION; ENDOCYTOSIS; ACTIVATION; INHIBITION;

D O I：

10.1371/journal.pone.0058395

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional and mechanistic studies of Wnt signaling have been severely hindered by the inaccessibility of bioactive proteins. To overcome this long-standing barrier, we engineered and characterized a panel of Chinese hamster ovary (CHO) cell lines with inducible transgenes encoding tagged and un-tagged human WNT1, WNT3A, WNT5A, WNT7A, WNT11, WNT16 or the soluble Wnt antagonist Fzd8CRD, all integrated into an identical genomic locus. Using a quantitative real-time bioluminescence assay, we show that cells expressing WNT1, 3A and 7A stimulate Wnt/beta-catenin reporter activity, while the other WNT expressing cell lines interfere with this activation. Additionally, in contrast to WNT3A, WNT1 only exhibits activity when cell-associated, and thus only signals to neighboring cells. The reporter assay also revealed a rapid decline of Wnt activity at 37 degrees C, indicating that Wnt activity is highly labile. These engineered cell lines will reduce the cost of making and purifying Wnt proteins and serve as a continuous, reliable and regulatable source of Wnts to research laboratories around the world.

引用

页数：11

共 44 条

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