Chromosome dynamic changes in two cultured chinese human embryonic stem cell lines: Single nucleotide polymorphism, copy number variation and loss of heterozygosity

被引:7
作者
Chen, Xue-Mei [1 ,2 ]
Kan, Quan-Cheng
Wang, Fang [1 ]
Kong, Hui-Juan [1 ]
Zhang, Yong-Yong [1 ]
Yu, Wen-Zhu [1 ]
Sun, Ying-Pu [1 ]
机构
[1] Zhengzhou Univ, Reprod Med Ctr, Affiliated Hosp 1, Zhengzhou 450052, Peoples R China
[2] Zhengzhou Univ, Dept Human Anat, Coll Basic Med Sci, Zhengzhou 450001, Peoples R China
基金
中国国家自然科学基金;
关键词
HUMAN EMBRYONIC STEM CELLS; COPY NUMBER VARIATION; SINGLE NUCLEOTIDE POLYMORPHISM; LOSS OF HETEROZYGOSITY; KARYOTYPE; IN-VITRO CULTURE; TUMORS; AMPLIFICATION; HYBRIDIZATION; INTEGRITY; REVEALS; GENES;
D O I
10.1002/jcb.24229
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The quality and safety of human embryonic stem cells (hESCs) in clinical application depend on gene stability. Two Chinese hESC lines, Zh1 and Zh21, were incubated over a long period. We observed and compared the gene stability in the passage numbers 20, 17 for Zh1 cell line and passage numbers 27, 60, 68 for Zh21 cell line. Single nucleotide polymorphisis analysis indicated that hESCs in early passages had relative gene stability; and with the increase in passage number, gene instability became strong. We also found that there were copy number variations (CNVs) in both Zh21 and Zh1. We analyzed the CNVs of Chinese Han Beijing man (CHB; normal Chinese people) and found that the all CNV forms were the loss in Zh21, Zh1, and CHB. We also analyzed and compared the related pathways of the mutant genes. We propose three steps to ensure hESC safety. Firstly, besides the conventional methods such as pluripotent genes, chromosome G-banding and teratoma, high-resolution DNA chip analysis should also be adopted; secondly, chromosomal properties are monitored every 10 passages in less than passage 50 and every 5 passages in more than passage 50; thirdly, the related pathways of mutant genes should be observed because only the mutant genes with variations of their related pathways may affected cell functions. J. Cell. Biochem. 113: 35203527, 2012. (C) 2012 Wiley Periodicals, Inc.
引用
收藏
页码:3520 / 3527
页数:8
相关论文
共 30 条
  • [1] Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
    Amps, Katherine
    Andrews, Peter W.
    Anyfantis, George
    Armstrong, Lyle
    Avery, Stuart
    Baharvand, Hossein
    Baker, Julie
    Baker, Duncan
    Munoz, Maria B.
    Beil, Stephen
    Benvenisty, Nissim
    Ben-Yosef, Dalit
    Biancotti, Juan-Carlos
    Bosman, Alexis
    Brena, Romulo Martin
    Brison, Daniel
    Caisander, Gunilla
    Camarasa, Maria V.
    Chen, Jieming
    Chiao, Eric
    Choi, Young Min
    Choo, Andre B. H.
    Collins, Daniel
    Colman, Alan
    Crook, Jeremy M.
    Daley, George Q.
    Dalton, Anne
    De Sousa, Paul A.
    Denning, Chris
    Downie, Janet
    Dvorak, Petr
    Montgomery, Karen D.
    Feki, Anis
    Ford, Angela
    Fox, Victoria
    Fraga, Ana M.
    Frumkin, Tzvia
    Ge, Lin
    Gokhale, Paul J.
    Golan-Lev, Tamar
    Gourabi, Hamid
    Gropp, Michal
    Lu Guangxiu
    Hampl, Ales
    Harron, Katie
    Healy, Lyn
    Herath, Wishva
    Holm, Frida
    Hovatta, Outi
    Hyllner, Johan
    [J]. NATURE BIOTECHNOLOGY, 2011, 29 (12) : 1132 - U113
  • [2] The inhibitor of apoptosis protein survivin is associated with high-risk behavior of neuroblastoma
    Azuhata, T
    Scott, D
    Takamizawa, S
    Wen, J
    Davidoff, A
    Fukuzawa, M
    Sandler, A
    [J]. JOURNAL OF PEDIATRIC SURGERY, 2001, 36 (12) : 1785 - 1791
  • [3] Adaptation to culture of human embryonic stem cells and oncogenesis in vivo
    Baker, Duncan E. C.
    Harrison, Neil J.
    Maltby, Edna
    Smith, Kath
    Moore, Harry D.
    Shaw, Pamela J.
    Heath, Paul R.
    Holden, Hazel
    Andrews, Peter W.
    [J]. NATURE BIOTECHNOLOGY, 2007, 25 (02) : 207 - 215
  • [4] Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture
    Caisander, G
    Park, H
    Frej, K
    Lindqvist, J
    Bergh, C
    Lundin, K
    Hanson, C
    [J]. CHROMOSOME RESEARCH, 2006, 14 (02) : 131 - 137
  • [5] SNPs Occur in Regions with Less Genomic Sequence Conservation
    Castle, John C.
    [J]. PLOS ONE, 2011, 6 (06):
  • [6] High-resolution analysis of the subtelomeric regions of human embryonic stem cells
    Darnfors, C
    Flodin, A
    Andersson, K
    Caisander, G
    Lindqvist, J
    Hyllner, J
    Wahlström, J
    Sartipy, P
    [J]. STEM CELLS, 2005, 23 (04) : 483 - 488
  • [7] Adjustment of genomic waves in signal intensities from whole-genome SNP genotyping platforms
    Diskin, Sharon J.
    Li, Mingyao
    Hou, Cuiping
    Yang, Shuzhang
    Glessner, Joseph
    Hakonarson, Hakon
    Bucan, Maja
    Maris, John M.
    Wang, Kai
    [J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (19)
  • [8] Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells
    Draper, JS
    Smith, K
    Gokhale, P
    Moore, HD
    Maltby, E
    Johnson, J
    Meisner, L
    Zwaka, TP
    Thomson, JA
    Andrews, PW
    [J]. NATURE BIOTECHNOLOGY, 2004, 22 (01) : 53 - 54
  • [9] Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells
    Enver, T
    Soneji, S
    Joshi, C
    Brown, J
    Iborra, F
    Orntoft, T
    Thykjaer, T
    Maltby, E
    Smith, K
    Abu Dawud, R
    Jones, M
    Matin, M
    Gokhale, P
    Draper, J
    Andrews, PW
    [J]. HUMAN MOLECULAR GENETICS, 2005, 14 (21) : 3129 - 3140
  • [10] In vitro culture conditions favoring selection of chromosomal abnormalities in human ES cells
    Imreh, M. P.
    Gertow, K.
    Cedervall, J.
    Unger, C.
    Holmberg, K.
    Szoke, K.
    Cosregh, L.
    Fried, G.
    Dilber, S.
    Blennow, E.
    Ahrlund-Richter, L.
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 99 (02) : 508 - 516