Raman microscopy for cellular investigations - From single cell imaging to drug carrier uptake visualization

被引:116
作者
Kann, Birthe [1 ,2 ]
Offerhaus, Herman L. [2 ]
Windbergs, Maike [1 ,3 ,4 ]
Otto, Cees [5 ]
机构
[1] Univ Saarland, Dept Biopharmaceut & Pharmaceut Technol, D-66123 Saarbrucken, Germany
[2] Univ Twente, Opt Sci Grp, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands
[3] Helmholtz Ctr Infect Res, D-66123 Saarbrucken, Germany
[4] Helmholtz Inst Pharmaceut Res Saarland, Dept Drug Delivery, D-66123 Saarbrucken, Germany
[5] Univ Twente, Med Cell BioPhys, MIRA Inst Biomed Technol & Tech Med, NL-7500 AE Enschede, Netherlands
关键词
Spontaneous Raman microscopy; Cell imaging; Drug carriers; Interaction between cell and carrier; Chemically selective visualization; Nanomaterials; Uptake; Intracellular fate of carriers; EMBRYONIC STEM-CELLS; BREAST-CANCER; LIVING CELLS; POLY(LACTIDE-CO-GLYCOLIDE) NANOPARTICLES; FLUORESCENCE MICROSCOPY; INTRACELLULAR DELIVERY; LIVE CELLS; PROTEIN DISTRIBUTION; GOLD NANOPARTICLES; EPITHELIAL-CELLS;
D O I
10.1016/j.addr.2015.02.006
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Progress in advanced therapeutic concepts requires the development of appropriate carrier systems for intracellular drug delivery. Consequently, analysis of interaction between carriers, drugs and cells as well as their uptake and intracellular fate is a current focus of research interest. In this context, Raman spectroscopy recently became an emerging analytical technique, due to its non-destructive, chemically selective and label-free working principle. In this review, we briefly present the state-of-the-art technologies for cell visualization and drug internalization. Against this background, Raman microscopy is introduced as a versatile analytical technique. An overview of various Raman spectroscopy investigations in this field is given including interactions of cells with drug molecules, carrier systems and other nanomaterials. Further, Raman instrumentations and sample preparation methods are discussed. Finally, as the analytical limit is not reached yet, a future perspective for Raman microscopy in pharmaceutical and biomedical research on the single cell level is given. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:71 / 90
页数:20
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