Molecular and Immunological Diagnostic Tests of COVID-19: Current Status and Challenges

被引：139
作者：
Kilic, Tugba [1 ,2 ]
Weissleder, Ralph [1 ,2 ,3 ]
Lee, Hakho [1 ,2 ]
机构：
[1] Harvard Med Sch, Ctr Syst Biol, Massachusetts Gen Hosp, Res Inst, Boston, MA 02114 USA
[2] Harvard Med Sch, Dept Radiol, Massachusetts Gen Hosp, Res Inst, Boston, MA 02114 USA
[3] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA
来源：
ISCIENCE | 2020年 / 23卷 / 08期
基金：
瑞士国家科学基金会;
关键词：
ACUTE RESPIRATORY SYNDROME; MEDIATED ISOTHERMAL AMPLIFICATION; I INFLUENZA-A; RAPID DETECTION; DIGITAL PCR; CORONAVIRUS; ASSAY; QUANTIFICATION; SARS-COV-2; SPECIMENS;
D O I：
10.1016/j.isci.2020.101406
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Rapid spread of coronavirus disease 2019 (COVID-19) is ravaging the globe. Since its first report in December 2019, COVID-19 cases have exploded to over 14 million as of July 2020, claiming more than 600,000 lives. Implementing fast and widespread diagnostic tests is paramount to contain COVID-19, given the current lack of an effective therapeutic or vaccine. This review focuses on a broad description of currently available diagnostic tests to detect either the virus (SARS-CoV-2) or virus-induced immune responses. We specifically explain the working mechanisms of these tests and compare their analytical performance. These analyses will assist in selecting most effective tests for a given application, for example, epidemiology or global pandemic research, population screening, hospital-based testing, home-based and point-of-care testing, and therapeutic trials. Finally, we lay out the shortcomings of certain tests and future needs.
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收藏
页数：19
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