The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro

被引：31

作者：

Yu, Yun ^{[1
]}

Lu, Yu ^{[2
]}

Bo, Ruonan ^{[1
]}

Huang, Yee ^{[1
]}

Hu, Yuanliang ^{[1
]}

Liu, Jiaguo ^{[1
]}

Wu, Yi ^{[1
]}

Tao, Yang ^{[1
]}

Wang, Deyun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2014年 / 460卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Gypenosides liposome; Optimization; Response surface methodology; Peritoneal macrophages; Phagocytic activity; Cytokine; EFFICIENCY; KINETICS; PROTEIN; DRUGS; CELLS;

D O I：

10.1016/j.ijpharm.2013.11.018

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Optimization of preparation of gypenosides liposome (GPSL) was investigated with response surface methodology (RSM) and the effect of GPSL activating phagocytosis activity and cytokine secretion of macrophages was determined in the paper. The results showed that the optimal conditions of GPSL preparation were as follows: the ratio of lipid to drug (w/w) of 8:1, the ratio of soybean phospholipid to cholesterol (w/w) of 6:1, the temperature of incubation of drug in water bath of 55 C, the time of incubation of drug of 11 min. With the condition, the experimental encapsulation efficiency of GPSL was 90.17 + 0.38%. In addition, GPSL could significantly enhance phagocytosis activity and the secretion of cytokines of the peritoneal macrophages of mice. These indicated that GPSL with high entrapping efficiency and small particle size could be prepared by ethanol injection combined with ammonium sulfate gradient method, which is applied easily, and GPSL could enhance the activity of peritoneal macrophages. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：248 / 254

页数：7

共 29 条

[1] Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-κB-mediated iNOS protein expression [J].