Detection and characterization of a lethal yellowing (16SrIV) group phytoplasma in Canary Island date palms affected by lethal decline in Texas

被引：78

作者：

Harrison, NA ^{[1
]}

Womack, M

Carpio, ML

机构：

[1] Univ Florida, Ft Lauderdale Res & Educ Ctr, Ft Lauderdale, FL 33314 USA

[2] Texas A&M Univ, Texas Agr Extens Serv, Robstown, TX 78380 USA

[3] Univ Florida, Ft Lauderdale Res & Educ Ctr, Ft Lauderdale, FL 33314 USA

来源：

PLANT DISEASE | 2002年 / 86卷 / 06期

关键词：

Arecaceae; phytoplasma identification;

D O I：

10.1094/PDIS.2002.86.6.676

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Polymerase chain reaction (PCR) assays were used to detect phytoplasmas in Canary Island date (Phoenix canariensis) palms displaying symptoms similar to lethal yellowing (LY) disease in Corpus Christi, TX. An rDNA product (1.8 kb) was amplified consistently from 10 of 11 palms by PCR employing phytoplasma universal rRNA primer pair P1/P7. Also, AluI endonuclease digests and sequencing of P1/P7 products revealed that nontarget Bacillus megaterium-related rDNA sequences of similar size were co-amplified along with phytoplasma rDNA from 10 palms. A 1,402-bp product was obtained from all 11 symptomatic palms when initial P1/P7 products were reamplified by PCR employing nested LY phytoplasma group-specific 16S rRNA primer pair LY16Sf/LY16Sr. Restriction fragment length polymorphism (RFLP) analysis of nested PCR products revealed that palm-infecting phytoplasmas were uniform and most similar to strains composing the coconut lethal yellowing phytoplasma (16SrIV) group. Sequence analysis of 16S rDNA determined the Texas Phoenix palm decline (TPD) phytoplasma to be phylogenetically closest to the Carludovica palmata leaf yellowing (CPY) phytoplasma. rDNA profiles of strains TPD and CPY obtained with AluI were co-identical and distinct from other known 16SrIV group phytoplasmas. On this basis, both strains were classified as members of a new subgroup, 16SrIV-D.

引用

页码：676 / 681

页数：6

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