Histamine iontophoresis on the viability of random skin flap in rats

被引:10
作者
Esteves Junior, Ivaldo [1 ,2 ]
Tacani, Pascale Mutti [3 ]
Liggieri, Victor Cicone
Ruggi, Bruno Grinman
Ferreira, Lydia Masako [4 ,5 ]
Liebano, Richard Eloin [6 ]
机构
[1] Univ Paulista, Phys Therapy Dept, Sao Paulo, Brazil
[2] Univ Prebiteriana Mackenzie, Sao Paulo, Brazil
[3] City Univ Sao Caetano do Sul, Phys Therapy Dept, USCS, Sao Paulo, Brazil
[4] Univ Fed Sao Paulo, Plast Div, Dept Surg, Sao Paulo, Brazil
[5] Univ Fed Sao Paulo, Postgrad Program Plast Surg, Sao Paulo, Brazil
[6] Univ Cidade Sao Paulo, UNICID, Phys Therapy Dept, Sao Paulo, Brazil
关键词
Histamine; Iontophoresis; Surgical Flaps; Necrosis; Rats; ELECTRICAL NERVE-STIMULATION; GENE-RELATED PEPTIDE; MUSCULOCUTANEOUS FLAP; SURVIVAL; TENS; AGENTS;
D O I
10.1590/S0102-86502009000100010
中图分类号
R61 [外科手术学];
学科分类号
摘要
Purpose: To evaluate the effects of the histamine iontophoresis on the random skin flap viability in rats. Methods: Sixty adult male Wistar rats were used. A cranially-based dorsal skin flap measuring 10 x 4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into four groups (G1-G4) (n=15 each group) as follows: G1 (control) - sham electrical stimulation, G2 (electrical stimulation) - direct current electrical stimulation, G3 (histamine) - histamine and sham electrical stimulation and G4 (histamine iontophoresis) - transdermal iontophoresis of histamine. In all groups the procedures were performed immediately after the surgery and on the two subsequent days. The percentage of flap necrosis was measured on the seventh postoperative day. Results: The mean and the respective standard deviation of the percentage of flap necrosis areas were as follows: G1 (control) - 47.87 +/- 9.13%, G2 - 51.49 +/- 8.19%, G3 - 46.33 +/- 8.32% and G4 - 30.82 +/- 11.25%. The G4 group presented a significantly smaller amount of flap necrosis when compared to the other groups (p<0.001). Conclusion: The topical administration of the histamine by iontophoresis was effective to increase the viability of the random skin flaps in rats.
引用
收藏
页码:48 / 51
页数:4
相关论文
共 21 条
[1]  
ABRAMSON D I, 1967, Archives of Physical Medicine and Rehabilitation, V48, P583
[2]  
[Anonymous], REV BRAS FISIOTER
[3]  
ASAI A, 1997, ANN PLAST SURG, V38, P514
[4]  
BRANCO CH, 2005, REV I CIENC SAUDE, V23, P11
[5]  
Esteves Junior Ivaldo, 2004, Acta Cir. Bras., V19, P626, DOI 10.1590/S0102-86502004000600008
[6]  
FINSETH F, 1978, PLAST RECONSTR SURG, V62, P738
[7]   Spinal cord stimulation improves survival in ischemic skin flaps: An experimental study of the possible mediation by calcitonin gene-related peptide [J].
Gherardini, G ;
Lundeberg, T ;
Cui, JG ;
Eriksson, SV ;
Trubek, S ;
Linderoth, B .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1999, 103 (04) :1221-1228
[8]   Histamine response and local cooling in the human skin:: involvement of H1- and H2-receptors [J].
Grossmann, M ;
Jamieson, MJ ;
Kirch, W .
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1999, 48 (02) :216-222
[9]  
Esteves Junior Ivaldo, 2005, Acta Cir. Bras., V20, P164, DOI 10.1590/S0102-86502005000200011
[10]   SKIN FLAP FAILURE - PATHO-PHYSIOLOGY [J].
KERRIGAN, CL .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1983, 72 (06) :766-774