Covalent Immobilization of Burkholderia Cepacia Lipase on Amine Functionalized Ionic Liquid Modified SBA-15

被引:9
作者
Hu Yi [1 ,2 ]
Yang Jiao [1 ]
Tang Su-Su [1 ]
Chu Xu-Ming [1 ]
Zou Bin [1 ]
Huang He [1 ,2 ]
机构
[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China
[2] Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China
来源
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2013年 / 34卷 / 05期
关键词
Burkholderia cepacia lipase; Ionic liquid; Mesoporous material; Modification; Immobilization; MESOPOROUS SILICA SBA-15; ADSORPTION; CATALYSTS; EFFICIENT;
D O I
10.7503/cjcu20120951
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The (3-aminopropyl) trimethoxysilane and amine functionalized ionic liquid modified mesoporous silica SBA-15(NH2-SBA, NH2-IL-SBA) were synthesized, and NH2-IL-SBA was activated by glutaraldehyde (CA-NH2-IL-SBA). Physical and chemical properties of the materials were characterized by elemental analysis, nitrogen adsorption-desorption, small-angle X-ray diffraction and Fourier transform infrared spectroscopy. The prepared materials were used as novel carrier systems to immobilize Burkholderia cepacia lipase(BCL) by physical adsorption, conventional covalent attachment and enzyme-aggregate coating methods. Enzymatic properties, including activity, optimum reaction condition and stability were investigated in the triacetin hydrolysis reaction. The results showed that the modification and activation did not destroy the structure of SBA-15. Compared with the immobilized BCL on NH2-SBA and parent SBA-15 (BCL-NH2-SBA, BCL-SBA-15), the immobilized BCL on novel carriers were more resistant to temperature and low pH changes and possessed both higher activity and stability. Especially, the BCL prepared by enzyme-aggregate coating method exhibited highest immobilization efficiency and stability, which improved 4.0 and 2.0 folds of thermal stability and reusability than BCL-SBA-15, respectively.
引用
收藏
页码:1195 / 1202
页数:8
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