LOOP-MEDIATED ISOTHERMAL AMPLIFICATION AS A SIMPLE MOLECULAR METHOD FOR THE DETECTION OF DERZSY'S DISEASE VIRUS

被引：6
作者：
Tarasiuk, Karolina [1 ]
Wozniakowski, Grzegorz [1 ]
Samorek-Salamonowicz, Elzbieta [1 ]
机构：
[1] Natl Inst Vet Res, Dept Poultry Viral Dis, PL-24100 Pulawy, Poland
来源：
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2013年 / 57卷 / 01期
关键词：
goose parvovirus; loop-mediated isothermal amplification; PCR; GOOSE-PARVOVIRUS; ASSAY; ANTIBODIES; FIELD;
D O I：
10.2478/bvip-2013-0004
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The objective of the study was to develop a simple and rapid molecular method for the detection of GPV. Twenty seven goose parvovirus (GPV) isolates collected from geese flocks in Poland were examined. Three pairs of specific primers: two outer primers (F3 and B3), two inner primers (FIP and BIP), and two loop primers (FL and BL) were used to accelerate the reaction. The optimum temperature and time of the reaction were 60 degrees C and 30 min. The sensitivity of the method was 10-times higher than PCR. The method proved to be a sensitive, rapid, and specific assay for detecting GPV.
引用
收藏
页码：19 / 23
页数：5
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