Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture

被引：24

作者：

de Souza, Gezimar D. ^{[1
,2
,3
]}

Mithoefer, Axel ^{[2
]}

Daolio, Cristina ^{[4
,5
]}

Schneider, Bernd ^{[4
]}

Rodrigues-Filho, Edson ^{[1
]}

机构：

[1] Univ Fed Sao Carlos UFSCar, Lab Bioquim Micromol Microorganism, Dept Quim, Sao Carlos, SP, Brazil

[2] Max Planck Inst Chem Ecol, Dept Bioorgan Chem, D-07745 Jena, Germany

[3] Accert Chem & Biotechnol Inc, Sao Carlos, SP, Brazil

[4] Max Planck Inst Chem Ecol, Biosynth NMR Res Grp, D-07745 Jena, Germany

[5] Bruker Biospin GmbH, NMR Applicat Grp, D-76287 Rheinstetten, Germany

来源：

MOLECULES | 2013年 / 18卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

LC-NMR; LC-SPE-NMR; mass spectrometry; alternariol; Alternaria; soybean; Glycine max; cell cultures; cytotoxicity; NUCLEAR-MAGNETIC-RESONANCE; MASS-SPECTROMETRY; MONOMETHYL ETHER; BROWN SPOT; MAMMALIAN-CELLS; MUTAGENICITY; PRODUCTS; CITRUS; SPECTROSCOPY; HPLC;

D O I：

10.3390/molecules18032528

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC50 values which range from 0.11 (+/- 0.02) to 4.69 (+/- 0.47) mu M showed the high cytotoxicity of these compounds.

引用

页码：2528 / 2538

页数：11

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