Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302

被引：18

作者：

Li, Lei ^{[1
]}

Chen, Xiaochun ^{[1
]}

Cheng, Jian ^{[1
]}

Zhang, Chunwei ^{[1
]}

Bai, Jianxin ^{[1
]}

Chen, Yong ^{[1
]}

Niu, Huanqin ^{[1
]}

Ying, Hanjie ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Life Sci & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2012年 / 35卷 / 08期

关键词：

Arthrobacter A302; Cyclic adenosine monophosphate; Dissolved oxygen; Two-stage oxygen supply control strategy; FERMENTATION; ACID; AGITATION; TENSION; CAMP; GUM;

D O I：

10.1007/s00449-012-0715-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Experiments confirmed dissolved oxygen (DO) definitely affects cyclic adenosine monophosphate (cAMP) production by Arthrobacter A302. Production of cAMP by batch fermentation was investigated under various DO conditions. A two-stage DO control strategy was proposed to achieve optimal production of cAMP based on the kinetic analysis: the DO level was controlled at 40% during the first 18 h and then switched to 30%. Relatively high cAMP production (9.9 g L-1) was achieved by applying this strategy. The cAMP productivity (0.14 g L-1 h(-1)) was also successfully improved by 85.1, 59.3, 15.1 and 28.0%, compared to cases in which DO was uncontrolled or DO levels were held at 20, 30 and 40%, respectively. This is the first report of the use of a two-stage DO control strategy in cAMP production, and it was verified to be an effective method for enhancing the cAMP yield via this strain.

引用

页码：1281 / 1286

页数：6

共 26 条

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