The draft genome of sweet orange (Citrus sinensis)

被引：791

作者：

Xu, Qiang ^{[1
]}

Chen, Ling-Ling ^{[2
]}

Ruan, Xiaoan ^{[3
]}

Chen, Dijun ^{[2
]}

Zhu, Andan ^{[1
]}

Chen, Chunli ^{[1
,2
]}

Bertrand, Denis ^{[3
]}

Jiao, Wen-Biao ^{[2
]}

Hao, Bao-Hai ^{[2
]}

Lyon, Matthew P. ^{[4
]}

Chen, Jiongjiong ^{[1
]}

Gao, Song ^{[3
]}

Xing, Feng ^{[2
]}

Lan, Hong ^{[1
]}

Chang, Ji-Wei ^{[2
]}

Ge, Xianhong ^{[5
]}

Lei, Yang ^{[2
]}

Hu, Qun ^{[1
]}

Miao, Yin ^{[2
]}

Wang, Lun ^{[1
]}

Xiao, Shixin ^{[1
]}

Biswas, Manosh Kumar ^{[1
]}

Zeng, Wenfang ^{[1
]}

Guo, Fei ^{[1
]}

Cao, Hongbo ^{[1
]}

Yang, Xiaoming ^{[1
]}

Xu, Xi-Wen ^{[2
]}

Cheng, Yun-Jiang ^{[1
]}

Xu, Juan ^{[1
]}

Liu, Ji-Hong ^{[1
]}

Luo, Oscar Junhong ^{[3
]}

Tang, Zhonghui ^{[2
]}

Guo, Wen-Wu ^{[1
]}

Kuang, Hanhui ^{[1
]}

Zhang, Hong-Yu ^{[2
]}

Roose, Mikeal L. ^{[4
]}

Nagarajan, Niranjan ^{[3
]}

Deng, Xiu-Xin ^{[1
]}

Ruan, Yijun ^{[2
,3
]}

机构：

[1] Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan, Peoples R China

[2] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China

[3] Genome Inst Singapore, Singapore, Singapore

[4] Univ Calif Riverside, Riverside, CA 92521 USA

[5] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China

来源：

NATURE GENETICS | 2013年 / 45卷 / 01期

基金：

中国国家自然科学基金;

关键词：

LTR-RETROTRANSPOSONS; MOLECULAR MARKERS; RNA-SEQ; SEQUENCE; FRUIT; GENE; PLANTS; ORIGIN; IDENTIFICATION; ALIGNMENT;

D O I：

10.1038/ng.2472

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Oranges are an important nutritional source for human health and have immense economic value. Here we present a comprehensive analysis of the draft genome of sweet orange (Citrus sinensis). The assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. We predicted 29,445 protein-coding genes, half of which are in the heterozygous state. With additional sequencing of two more citrus species and comparative analyses of seven citrus genomes, we present evidence to suggest that sweet orange originated from a backcross hybrid between pummelo and mandarin. Focused analysis on genes involved in vitamin C metabolism showed that GalUR, encoding the rate-limiting enzyme of the galacturonate pathway, is significantly upregulated in orange fruit, and the recent expansion of this gene family may provide a genomic basis. This draft genome represents a valuable resource for understanding and improving many important citrus traits in the future.

引用

页码：59 / U92

页数：10

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