Nuclear Hormone Receptor Expression in Mouse Kidney and Renal Cell Lines

被引：13

作者：

Ogawa, Daisuke ^{[1
,2
]}

Eguchi, Jun ^{[1
]}

Wada, Jun ^{[1
]}

Terami, Naoto ^{[1
]}

Hatanaka, Takashi ^{[1
]}

Tachibana, Hiromi ^{[1
]}

Nakatsuka, Atsuko ^{[1
,2
]}

Horiguchi, Chikage Sato ^{[1
]}

Nishii, Naoko ^{[1
]}

Makino, Hirofumi ^{[1
]}

机构：

[1] Okayama Univ Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci, Okayama, Japan

[2] Okayama Univ Grad Sch Med Dent & Pharmaceut Sci, Dept Diabet Nephropathy, Okayama, Japan

来源：

PLOS ONE | 2014年 / 9卷 / 01期

关键词：

PPAR-ALPHA AGONIST; DIABETIC-NEPHROPATHY; ERR-ALPHA; INFLAMMATION; ACTIVATION; MICE; FENOFIBRATE; PROTEINURIA; METABOLISM; FIBROSIS;

D O I：

10.1371/journal.pone.0085594

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nuclear hormone receptors (NHRs) are transcription factors that regulate carbohydrate and lipid metabolism, immune responses, and inflammation. Although several NHRs, including peroxisome proliferator-activated receptor-gamma (PPAR gamma) and PPAR alpha, demonstrate a renoprotective effect in the context of diabetic nephropathy (DN), the expression and role of other NHRs in the kidney are still unrecognized. To investigate potential roles of NHRs in the biology of the kidney, we used quantitative real-time polymerase chain reaction to profile the expression of all 49 members of the mouse NHR superfamily in mouse kidney tissue (C57BL/6 and db/m), and cell lines of mesangial (MES13), podocyte (MPC), proximal tubular epithelial (mProx24) and collecting duct (mIMCD3) origins in both normal and high-glucose conditions. In C57BL/6 mouse kidney cells, hepatocyte nuclear factor 4 alpha, chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) and COUP-TFIII were highly expressed. During hyperglycemia, the expression of the NHR 4A subgroup including neuron-derived clone 77 (Nur77), nuclear receptor-related factor 1, and neuron-derived orphan receptor 1 significantly increased in diabetic C57BL/6 and db/db mice. In renal cell lines, PPAR delta was highly expressed in mesangial and proximal tubular epithelial cells, while COUP-TFs were highly expressed in podocytes, proximal tubular epithelial cells, and collecting duct cells. High-glucose conditions increased the expression of Nur77 in mesangial and collecting duct cells, and liver x receptor alpha in podocytes. These data demonstrate NHR expression in mouse kidney cells and cultured renal cell lines and suggest potential therapeutic targets in the kidney for the treatment of DN.

引用

页数：12

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