Binding of Clostridium perfringens to collagen correlates with the ability to cause necrotic enteritis in chickens

被引：71
作者：
Wade, B. [1 ,2 ]
Keyburn, A. L. [1 ,2 ]
Seemann, T. [2 ,3 ]
Rood, J. I. [2 ]
Moore, R. J. [1 ,2 ,4 ]
机构：
[1] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia
[2] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia
[3] Univ Melbourne, Victorian Life Sci Computat Initiat, Parkville, Vic 3010, Australia
[4] RMIT Univ, Sch Appl Sci, Bundoora, Vic 3083, Australia
来源：
VETERINARY MICROBIOLOGY | 2015年 / 180卷 / 3-4期
关键词：
Clostridium perfringens; Necrotic enteritis; Adhesion; Adhesin; Collagen; Poultry; ADHESIVE PROPERTIES; IDENTIFICATION; TOXIN; ADHERENCE; PROTEINS; DISEASE; REGIONS;
D O I：
10.1016/j.vetmic.2015.09.019
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
This study investigated the ability of Clostridium perfringens isolates derived from chickens to bind to collagen types I-V and gelatin. In total 21 strains from three distinct backgrounds were studied: (i) virulent strains isolated from birds suffering from necrotic enteritis, (ii) avirulent strains isolated from birds suffering from necrotic enteritis and (iii) strains isolated from healthy birds. All strains isolated from diseased birds had been assessed for virulence in a disease induction model. The virulent isolates all displayed collagen binding ability. However, most strains in the other two classes showed negligible binding to collagen. The prevalence of a previously described C perfringens putative collagen adhesin-encoding gene was investigated by PCR screening. It was found that five of the strains carried the putative collagen adhesin-encoding gene and that all of these strains were virulent isolates. Based on these studies it is postulated that collagen adhesion may play a role in the pathogenesis of necrotic enteritis. Crown Copyright (C) 2015 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：299 / 303
页数：5
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