Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode

被引：83

作者：

Lin, Jingyu ^{[1
]}

Mazarei, Mitra ^{[1
]}

Zhao, Nan ^{[1
]}

Zhu, Junwei J. ^{[2
]}

Zhuang, Xiaofeng ^{[1
]}

Liu, Wusheng ^{[1
]}

Pantalone, Vincent R. ^{[1
]}

Arelli, Prakash R. ^{[3
]}

Stewart, Charles N., Jr. ^{[1
]}

Chen, Feng ^{[1
]}

机构：

[1] Univ Tennessee, Dept Plant Sci, Knoxville, TN USA

[2] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA

[3] USDA ARS, Crop Genet Res Unit, Jackson, TN USA

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2013年 / 11卷 / 09期

基金：

美国国家科学基金会;

关键词：

methyl salicylate; transgenic hairy roots; soybean; Heterodera glycines; FLORAL SCENT PRODUCTION; CARBOXYL METHYLTRANSFERASE; HETERODERA-GLYCINES; METHYL SALICYLATE; INDOLE-3-ACETIC-ACID METHYLTRANSFERASE; ARABIDOPSIS-THALIANA; MICROARRAY ANALYSIS; RHG1; LOCUS; EXPRESSION; BIOSYNTHESIS;

D O I：

10.1111/pbi.12108

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Salicylic acid plays a critical role in activating plant defence responses after pathogen attack. Salicylic acid methyltransferase (SAMT) modulates the level of salicylic acid by converting salicylic acid to methyl salicylate. Here, we report that a SAMT gene from soybean (GmSAMT1) plays a role in soybean defence against soybean cyst nematode (Heterodera glycines Ichinohe, SCN). GmSAMT1 was identified as a candidate SCN defence-related gene in our previous analysis of soybean defence against SCN using GeneChip microarray experiments. The current study started with the isolation of the full-length cDNAs of GmSAMT1 from a SCN-resistant soybean line and from a SCN-susceptible soybean line. The two cDNAs encode proteins of identical sequences. The GmSAMT1 cDNA was expressed in Escherichia coli. Using in vitro enzyme assays, E.coli-expressed GmSAMT1 was confirmed to function as salicylic acid methyltransferase. The apparent Km value of GmSAMT1 for salicylic acid was approximately 46m. To determine the role of GmSAMT1 in soybean defence against SCN, transgenic hairy roots overexpressing GmSAMT1 were produced and tested for SCN resistance. Overexpression of GmSAMT1 in SCN-susceptible backgrounds significantly reduced the development of SCN, indicating that overexpression of GmSAMT1 in the transgenic hairy root system could confer resistance to SCN. Overexpression of GmSAMT1 in transgenic hairy roots was also found to affect the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction.

引用

页码：1135 / 1145

页数：11

共 53 条

[1] Diversity and Evolution of Coral Fluorescent Proteins [J].