Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

被引：17

作者：

Kim, Song Yee ^{[1
]}

Kang, Young Ae ^{[1
]}

Bae, Il Kwon ^{[2
,3
]}

Yim, Jae-Joon ^{[4
,5
]}

Park, Moo Suk ^{[1
]}

Kim, Young Sam ^{[1
]}

Kim, Se Kyu ^{[1
]}

Chang, Joon ^{[1
]}

Jeong, Seok Hoon ^{[2
,3
]}

机构：

[1] Yonsei Univ, Coll Med, Div Pulmonol, Dept Internal Med, Seoul 120752, South Korea

[2] Yonsei Univ, Coll Med, Dept Lab Med, Seoul 135720, South Korea

[3] Yonsei Univ, Coll Med, Res Inst Bacterial Resistance, Seoul 135720, South Korea

[4] Seoul Natl Univ, Coll Med, Div Pulm & Crit Care Med, Dept Internal Med, Seoul 110744, South Korea

[5] Seoul Natl Univ, Coll Med, Lung Inst, Seoul 110744, South Korea

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 77卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Mycobacterium abscessus; Mycobacterium massiliense; Multilocus sequence analysis; rpoB; Multilocus sequence typing; RAPIDLY GROWING MYCOBACTERIA; FIELD GEL-ELECTROPHORESIS; NONTUBERCULOUS MYCOBACTERIA; AVIUM COMPLEX; KLEBSIELLA-PNEUMONIAE; SP-NOV; IDENTIFICATION; INFECTION; OUTBREAK; PREVALENCE;

D O I：

10.1016/j.diagmicrobio.2013.06.023

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：143 / 149

页数：7

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