14α-Hydroxylation of steroids by mycelium of the mold fungus Curvularia lunata (VKPM F-981) to produce precursors for synthesizing new steroidal drugs

被引：20

作者：

Andryushina, V. A. ^{[1
]}

Voishvillo, N. E. ^{[1
]}

Druzhinina, A. V. ^{[1
]}

Stytsenko, T. S. ^{[1
]}

Yaderets, V. V. ^{[1
]}

Petrosyan, M. A. ^{[2
]}

Zeinalov, O. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Ctr Bioengn, Moscow 117312, Russia

[2] Russian Acad Med Sci, Res Inst Obstet & Gynecol, St Petersburg 199034, Russia

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2013年 / 47卷 / 02期

关键词：

14 alpha-hydroxysteroid derivatives; Curvularia lunata; hydroxylation; TRANSFORMATION; HYDROXYLATION;

D O I：

10.1007/s11094-013-0905-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Five 14 alpha-hydroxylated derivatives of androstane and pregnane steroids were obtained using mycelium of the mold fungus Curvularia lunata (VKPM F-981). The conditions for microbiological transformation of androst-4-en-3,17-dione that enabled its 14 alpha-hydroxy analog to be obtained in yields up to 60% with substrate loading 6 g/L were determined. The 21-acetoxy analog of proligestone was synthesized from 14 alpha-hydroxycortexolone that was formed simultaneously with hydrocortisone during hydroxylation of cortexolone by C. lunata. The resulting 14 alpha- and 14 alpha,21-hydroxysteroids could be used as precursors for the synthesis of new drugs.

引用

页码：103 / 108

页数：6

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