Determination of the 'apparent pKa' of selected food hydrocolloids using ortho-toluidine blue

被引:36
作者
Vleugels, Leo F. W. [1 ,2 ]
Ricois, Stella [3 ]
Voets, Ilja K. [1 ,2 ]
Tuinier, Remco [1 ,2 ]
机构
[1] Eindhoven Univ Technol, Dept Chem Engn & Chem, Lab Phys Chem, POB 513, NL-5600 MB Eindhoven, Netherlands
[2] Eindhoven Univ Technol, Inst Complex Mol Syst, POB 513, NL-5600 MB Eindhoven, Netherlands
[3] DSM Mat Sci Ctr, T&C, Urmonderbaan 22, NL-6160 MD Geleen, Netherlands
关键词
Polysaccharides; Ortho-toluidine blue; Metachromasy; Apparent pK(a); Polyelectrolyte behaviour; Low ionic strength; ACID-BASE EQUILIBRIA; PROTON DISSOCIATION; IONIC-STRENGTH; POLYELECTROLYTES; ALGINATE; BEHAVIOR; METACHROMASY; COUNTERIONS; MOBILITY; CALCIUM;
D O I
10.1016/j.foodhyd.2018.02.049
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Charged food hydrocolloids provide structure and texture in manufactured foods by their organization and electrostatic interactions with other species and small ions. These electrostatic interactions depend on their actual charge density which cannot be predicted from pK(a) values of weakly charged monomers. In practice, this is circumvented by the use of an 'apparent pK(a)' (pK(a) (app)) which depends on solution ionic strength and type of counter-ions. pK(a) (app) values for food hydrocolloids are poorly documented, especially in low ionic strength solvents. Titration of a metachromatic alginate-ortho-toluidine blue complex revealed a sigmoidal, pH-dependent, dye metachromasy response with its centre located at pH = pK(a) ((app)). To test the suitability of this approach for the determination of pK(a) ((app)), five food hydrocolloids and a synthetic polyanion are analysed and the results are compared to classical methods such as potentiometric titration. Metachromasy is shown to occur when the polymer inter-charge distance is 4.5 angstrom; the complex stoichiometry depending on inter-charge distance. pK(a) ((app)) of stoichiometric complexes at low ionic strength (1 mM phosphate buffer) were successfully determined (alginate 4.5, sodium carboxymethylcellulose 4.6, k-carrageenan 1.2, polyphosphate 2.1 and polyacrylic acid 5.8) and are comparable to values obtained by potentiometric titration. Further, it is shown that pK(a) ((app)) determination by metachromatic dye titration is feasible for stoichiometric polymer-dye complexes at low concentrations (20-200 mu M) in low ionic strength (< 10 mM) aqueous solutions. Unlike classical methods, the metachromatic dye titration approach is suitable for low polymer concentrations in aqueous solutions at low ionic strength. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:273 / 283
页数:11
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