Biologically-active unilamellar vesicles from red blood cells

被引:4
作者
Jang, Hyun-Sook [1 ]
Cho, Yoon-Kyoung [1 ,2 ]
Granick, Steve [1 ,3 ]
机构
[1] Inst Basic Sci IBS, Ctr Soft & Living Matter, Ulsan 44919, South Korea
[2] Ulsan Natl Inst Sci & Technol UNIST, Dept Biomed Engn, Ulsan 44919, South Korea
[3] Ulsan Natl Inst Sci & Technol UNIST, Dept Chem, Ulsan 44919, South Korea
关键词
MEMBRANE-COATED NANOPARTICLES; GLUCOSE-TRANSPORTER; GIANT LIPOSOMES; RECONSTITUTION; AGAROSE; FILMS;
D O I
10.1039/c8bm01461b
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
We demonstrate a method to prepare giant unilamellar vesicles (GUVs) with biologically-active protein activity, by mixing erythrocyte (red blood cell) membrane extract with phospholipids and growing their mixture in a porous hydrogel matrix. This presents a pathway to retain protein biological activity without prior isolation and purification of the protein, though only the activity of the membrane protein GLUT1 is investigated to date. Using the cascade enzymatic reaction glucose oxidase and horseradish peroxidase to assay glucose concentration specifically within the GUV interior, we show that glucose is internalized by GLUT1 whereas adding cytochalasin B, a GLUT1 inhibitor, blocks glucose transport. The method presented here operates at biological ionic strength and is both simple and potentially generalizable.
引用
收藏
页码:1393 / 1398
页数:6
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