Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

被引：24

作者：

Huang, Y. C. ^{[1
]}

Tzeng, W. S. ^{[3
,5
,6
]}

Wang, C. C. ^{[4
,7
]}

Cheng, B. C. ^{[4
,8
]}

Chang, Y. K. ^{[1
]}

Chen, H. H. ^{[3
]}

Lin, P. C. ^{[1
]}

Huang, T. Y. ^{[1
]}

Chuang, T. J. ^{[2
]}

Lin, J. W. ^{[9
,10
]}

Chang, C. P. ^{[8
]}

机构：

[1] Chi Mei Med Ctr, Dept Radiol, Tainan, Taiwan

[2] Chi Mei Med Ctr, Dept Intens Care Med, Tainan, Taiwan

[3] Chi Mei Med Ctr, Dept Med Imaging, Tainan, Taiwan

[4] Chi Mei Med Ctr, Dept Surg, Tainan, Taiwan

[5] Cent Taiwan Univ Sci & Technol, Dept Radiol Technol, Taichung, Taiwan

[6] Chia Nan Univ Pharm & Sci, Dept Informat Technol, Coll Hlth & Informat, Tainan, Taiwan

[7] Southern Taiwan Univ Sci & Technol, Dept Child Care, Tainan, Taiwan

[8] Southern Taiwan Univ Sci & Technol, Dept Biotechnol, Tainan, Taiwan

[9] Taipei Med Univ, Dept Neurosurg, Shuang Ho Hosp, Taipei, Taiwan

[10] Taipei Med Univ, Grad Inst Clin Med, Taipei, Taiwan

来源：

MAGNETIC RESONANCE IMAGING | 2013年 / 31卷 / 07期

关键词：

Stroke; Magnetic resonance image; Agmatine; Brain edema; Pharmacology; MAGNETIC-RESONANCE; HYPOXIA-ISCHEMIA; PRETERM INFANTS; CRANIAL ULTRASOUND; ARTERY OCCLUSION; FOCAL ISCHEMIA; ACUTE STROKE; BRAIN; MODEL; REPERFUSION;

D O I：

10.1016/j.mri.2013.03.026

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90 min, agmatine was injected 5 min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced TI-weighted images (CE-TI WI) during 3 h-72 h in a 1.5 T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-TI WI, and TTC all were noted at 3 h and peaked at 24 h-48 h after MCAO injury. 'ITC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-TI WI-derived lesion volumes at the last imaging time (72 h) point except for significantly smaller ADC lesions in the MCAO model (P < 0.05). Volumetric calculation based on TTCderived infarct also correlated significantly stronger to volumetric calculation based on last imaging time point derived on T2WI, DWI or CE-TI WI than ADC (P < 0.05). At the last imaging time point, a significant increase in Evans Blue extravasation and a significant decrease in Nissl-positive cells numbers were noted in the vehicle-treated MCAO injured animals. The lesion volumes derived from T2WI, DWI, CE-T1WI, and Evans blue extravasation as well as the reduced numbers of Nissl-positive cells were all significantly attenuated in the agmatine-treated rats compared with the control ischemia rats (P <0.05). Conclusion: Our results suggest that agmatine has neuroprotective effects against brain edema on a reperfusion model after transient cerebral ischemia. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：1174 / 1181

页数：8

共 41 条

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