Liposome-mediated DNA vaccination

被引：136

作者：

Gregoriadis, G ^{[1
]}

Saffie, R ^{[1
]}

deSouza, JB ^{[1
]}

机构：

[1] UCL, SCH MED, DEPT IMMUNOL, LONDON W1P 9PG, ENGLAND

来源：

FEBS LETTERS | 1997年 / 402卷 / 2-3期

关键词：

liposome; plasmid DNA; DNA vaccination;

D O I：

10.1016/S0014-5793(96)01507-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous reports have indicated that intramuscular injection of antigen-coding naked plasmid DNA can trigger humoral and cell-mediated protective immunity against infection. This follows DNA uptake by muscle fibres, leading to the expression and extracellular release of the antigen, Here it is shown for the first time that intramuscular immunization of mice with pRc/CMV HBS (encoding the S region of hepatitis B antigen; HBsAg) entrapped into positively charged (cationic) liposomes leads to greatly improved humoral and cell-mediated immunity. These cationic liposome-entrapped DNA vaccines generate titres of anti-HBsAg IgG(1) antibody isotype in excess of 100-fold higher and increased levels of both IFN-gamma and IL-4 when compared with naked DNA or DNA complexed with preformed similar (cationic) liposomes. It is likely that immunization with liposome-entrapped plasmid DNA involves antigen-presenting cells locally or in the regional draining lymph nodes.

引用

页码：107 / 110

页数：4

共 27 条

[1] BOVINE HERPESVIRUS-1 - IMMUNE-RESPONSES IN MICE AND CATTLE INJECTED WITH PLASMID DNA [J].