HIGH CONTENT SCREENING OF DEFINED CHEMICAL LIBRARIES USING NORMAL AND GLIOMA-DERIVED NEURAL STEM CELL LINES

被引：11

作者：

Danovi, Davide ^{[1
]}

Folarin, Amos A. ^{[1
]}

Baranowski, Bart ^{[1
]}

Pollard, Steven M. ^{[1
]}

机构：

[1] UCL, UCL Canc Inst, Dept Canc Biol, Samantha Dickson Brain Canc Unit, London, England

来源：

IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS: IMAGING LIVE CELLS IN HEALTH AND DISEASE | 2012年 / 506卷

关键词：

CENTRAL-NERVOUS-SYSTEM; IN-VITRO; DIFFERENTIATION CAPACITY; ADHERENT CULTURE; BRAIN-TUMORS; NS CELLS; ADULT; PHENOTYPE; NEURONS; FETAL;

D O I：

10.1016/B978-0-12-391856-7.00040-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small molecules with potent biological effects on the fate of normal and cancer-derived stem cells represent both useful research tools and new drug leads for regenerative medicine and oncology. Long-term expansion of mouse and human neural stem cells is possible using adherent monolayer culture. These cultures represent a useful cellular resource to carry out image-based high content screening of small chemical libraries. Improvements in automated microscopy, desktop computational power, and freely available image processing tools, now means that such chemical screens are realistic to undertake in individual academic laboratories. Here we outline a cost effective and versatile time lapse imaging strategy suitable for chemical screening. Protocols are described for the handling and screening of human fetal Neural Stem (NS) cell lines and their malignant counterparts, Glioblastoma-derived neural stem cells (GNS). We focus on identification of cytostatic and cytotoxic "hits" and discuss future possibilities and challenges for extending this approach to assay lineage commitment and differentiation.

引用

页码：311 / 329

页数：19

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