Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2

被引：27

作者：

Shang, Shufeng ^{[1
]}

Jiang, Jun ^{[1
]}

Deng, Yiqun ^{[1
]}

机构：

[1] South China Agr Univ, Guangdong Prov Key Lab Prot Funct & Regulat Agr O, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2013年 / 14卷 / 06期

基金：

中国国家自然科学基金;

关键词：

T-2; toxin; chicken CYP1A5; metabolism; EXPRESSION; CYP1A5; LIVER; RADIOACTIVITY; TRANSMISSION; DRUG; P450;

D O I：

10.3390/ijms140610809

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5 alpha further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.

引用

收藏

页码：10809 / 10818

页数：10

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