DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides

被引：123

作者：

Dowler, S ^{[1
]}

Currie, RA ^{[1
]}

Downes, CP ^{[1
]}

Alessi, DR ^{[1
]}

机构：

[1] Univ Dundee, Dept Biochem, MRC, Prot Phosphorylat Unit, Dundee DD1 5EH, Scotland

来源：

BIOCHEMICAL JOURNAL | 1999年 / 342卷

关键词：

DAPP; PI; 3-kinase; phosphoinositide; PH domain; SH2; domain;

D O I：

10.1042/0264-6021:3420007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by an Src homology (SH2) domain and a pleckstrin homology (PH) domain at its C-terminus. It has been termed dual adaptor for phosphotyrosine: and 3-phosphoinositides (DAPP1). DAPP1 is widely expressed and exhibits high-affinity interactions with PtdIns(3,4,5)P-3 and PtdIns(3,4)P-2, but not with other phospholipids tested. These observations predict that DAPP1 will interact with both tyrosine phosphorylated proteins and 3-phosphoinositides and may therefore play a role in regulating the location and/or activity of such proteins(s) in response to agonists that elevate PtdIns(3,4,5)P-3 and PtdIns(3,4)P-2.

引用

页码：7 / 12

页数：6

共 24 条

[1] Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha [J].

Alessi, DR ;

James, SR ;

Downes, CP ;

Holmes, AB ;

Gaffney, PRJ ;

Reese, CB ;

Cohen, P .

CURRENT BIOLOGY, 1997, 7 (04) :261-269

[2] The role of PI 3-kinase in insulin action [J].

Alessi, DR ;

Downes, CP .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1998, 1436 (1-2) :151-164

[3] 3-phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase [J].

Alessi, DR ;

Deak, M ;

Casamayor, A ;

Caudwell, FB ;

Morrice, N ;

Norman, DG ;

Gaffney, P ;

Reese, CB ;

MacDougall, CN ;

Harbison, D ;

Ashworth, A ;

Bownes, M .

CURRENT BIOLOGY, 1997, 7 (10) :776-789

[4] Mechanism of activation of protein kinase B by insulin and IGF-1 [J].

Alessi, DR ;

Andjelkovic, M ;

Caudwell, B ;

Cron, P ;

Morrice, N ;

Cohen, P ;

Hemmings, BA .

EMBO JOURNAL, 1996, 15 (23) :6541-6551

[5] Insulin signal transduction through protein kinase cascades [J].

Avruch, J .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1998, 182 (1-2) :31-48

[6] PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2 [J].

Balendran, A ;

Casamayor, A ;

Deak, M ;

Paterson, A ;

Gaffney, P ;

Currie, R ;

Downes, CP ;

Alessi, DR .

CURRENT BIOLOGY, 1999, 9 (08) :393-404

[7] Intracellular signalling: PDK1 - a kinase at the hub of things [J].

Belham, C ;

Wu, SL ;

Avruch, J .

CURRENT BIOLOGY, 1999, 9 (03) :R93-R96

[8] Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1 [J].

Currie, RA ;

Walker, KS ;

Gray, A ;

Deak, M ;

Casamayor, A ;

Downes, CP ;

Cohen, P ;

Alessi, DR ;

Lucocq, J .

BIOCHEMICAL JOURNAL, 1999, 337 :575-583

[9] Characterisation of a plant 3-phosphoinositide-dependent protein kinase-1 homologue which contains a pleckstrin homology domain [J].

Deak, M ;

Casamayor, A ;

Currie, RA ;

Downes, CP ;

Alessi, DR .

FEBS LETTERS, 1999, 451 (03) :220-226

[10] Synthesis of 1-O-stearoyl-2-O-arachidonoyl-sn-glycer-3-yl-D-myo-inositol 3,4,5-trisphosphate and its stereoisomers [J].

Gaffney, PRJ ;

Reese, CB .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1997, 7 (24) :3171-3176

← 1 2 3 →