The structure of the Escherichia coli EF-Tu center dot EF-Ts complex at 2.5 angstrom resolution

被引：284
作者：
Kawashima, T
BerthetColominas, C
Wulff, M
Cusack, S
Leberman, R
机构：
[1] EUROPEAN MOLEC BIOL LAB, GRENOBLE OUTSTN, F-38042 GRENOBLE, FRANCE
[2] EUROPEAN SYNCHROTRON RADIAT FACIL, F-38043 GRENOBLE, FRANCE
来源：
NATURE | 1996年 / 379卷 / 6565期
关键词：
D O I：
10.1038/379511a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The crystal structure of the EF-Tu . EF-Ts complex from Escherichia coli has been determined to a resolution of 2.5 Angstrom. The complex contains two subunits of each of the elongation factors. The two EF-Ts molecules form a tight dimer, but there is little contact between the two EF-Tu molecules. The interaction of EF-Ts with EF-Tu results principally in the disruption of the Mg2+ ion binding site, thereby reducing the affinity of EF-Tu for guanine nucleotides.
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页码：511 / 518
页数：8
相关论文
共 30 条
[1]   ORGANIZATION AND NUCLEOTIDE-SEQUENCE OF A NEW RIBOSOMAL OPERON IN ESCHERICHIA-COLI CONTAINING THE GENES FOR RIBOSOMAL-PROTEIN S2 AND ELONGATION-FACTOR TS [J].
AN, G ;
BENDIAK, DS ;
MAMELAK, LA ;
FRIESEN, JD .
NUCLEIC ACIDS RESEARCH, 1981, 9 (16) :4163-4172
[2]  
ARAI KI, 1972, J BIOL CHEM, V247, P7029
[3]   CRYSTAL-STRUCTURE OF ACTIVE ELONGATION-FACTOR TU REVEALS MAJOR DOMAIN REARRANGEMENTS [J].
BERCHTOLD, H ;
RESHETNIKOVA, L ;
REISER, COA ;
SCHIRMER, NK ;
SPRINZL, M ;
HILGENFELD, R .
NATURE, 1993, 365 (6442) :126-132
[4]   BACTERIOPHAGE QBETA REPLICASE CONTAINS PROTEIN-BIOSYNTHESIS ELONGATION FACTORS EF TU AND EF TS [J].
BLUMENTHAL, T ;
WEBER, K ;
LANDERS, TA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (05) :1313-+
[5]   RNA REPLICATION - FUNCTION AND STRUCTURE OF QBETA-REPLICASE [J].
BLUMENTHAL, T ;
CARMICHAEL, GG .
ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 :525-548
[6]   PROTEINS REGULATING RAS AND ITS RELATIVES [J].
BOGUSKI, MS ;
MCCORMICK, F .
NATURE, 1993, 366 (6456) :643-654
[7]  
BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
[8]   FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES [J].
BRUNGER, AT .
NATURE, 1992, 355 (6359) :472-475
[9]   ROLE OF GTP IN PROTEIN SYNTHESIS - INTERACTION OF GTP WITH SOLUBLE TRANSFER FACTORS FROM E COLI [J].
ERTEL, R ;
REDFIELD, B ;
BROT, N ;
WEISSBACH, H .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 128 (02) :331-+
[10]  
JANSSEN GMC, 1994, J BIOL CHEM, V269, P31410
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